The Molecular Genetics Core facility is approximately 7,500 square feet. There are two large laboratories (~1500 sq. ft. each), a DNA sequencing and genotyping room (~800 sq. ft.), two cold rooms, dark room, and a walk-in freezer. The laboratory space is equipped with benches, tables, sinks, distilled water, fume hoods and separate areas for tissue culture, PCR, and radioisotope use. Four Pentium III NT Workstations (400-500 MHz) and four Power Macintosh G4’s provide the computational hardware for the Molecular Genetics laboratory. The eight computers are networked together via the Genome Center NT server with the 5 computers operating three ABI 377 DNA sequencers and two DNA Wave Machines in addition to the computers used by the Genetic Epidemiology and Statistical Genetics units. The molecular genetics laboratory contains all the standard equipment necessary for large-scale, high throughput molecular analysis of DNA variation. These items include centrifuges, water baths, gel electrophoresis apparatus, pipettes, glassware, balances, etc. The laboratory also has two Transgenomics DNA Wave machines for SNP detection using dHPLC. The genotyping room contains three ABI 377 automated sequencers, ten Perkin Elmer 9700 thermocyclers, and the PSQ 96 Pyrosequencing platform for SNP genotyping. Molecular genetics laboratory space on the 5th floor of the Cancer Center contains two ABI 373 automated sequencers. The immunogenetics core research laboratory, also on the 5 th floor of the cancer center, provides approximately 800 sq ft of additional laboratory space for molecular genetics work. The Genome Center also contains functional assays, mutation detection, and positional cloning, and a separate tissue culture facility for extraction of DNA from blood samples and EBV cell transformation. Also available are industrial freezers, refrigerators, centrifuges and a dark room, and an electron microscope.