• Item: Clear Microtitre Plates | Creatinine Standard |  StressXpress®Creatinine Reagent | Plate Sealer
• Target: Urine Creatinine
• Utility: Detection kit used to quantitatively measure creatinine in samples.
• Category: Assay Kits
• Platform: Microplate
• Precision: Intra Assay Precision: Three human urine samples were diluted 1:20 with deionized water and run in replicates of 20 in an assay. The mean and precision of the calculated creatinine concentrations were: Sample 1- 1174.3 pg/mL, 6% CV Sample 2- 475.9 pg/mL, 5.6% CV Sample 3- 177.4 pg/mL, 14.7% CV Inter Assay Precision: Three human urine samples were diluted 1:20 with deionized water and run in duplicates in 20 assays run over five days by three operators. The mean and precision of the calculated creatinine concentrations were: Sample 1- 1188.1 pg/mL, 7.2% CV Sample 2- 508.7 pg/mL, 6.3% CV Sample 3- 199.7 pg/mL, 10.9% CV
• Assay Type: Direct Quantitative Assay
• References: 1. Wallimann, T. et al., Biochem. J., 2000, 281, 21-40. 2. Wyss, M. and Kaddurah-Daouk, R., Physiol. Rev., 2000, 80, 1107-1213. 3. Raja Iyengar, M. et al., J. Biol. Chem, 1985, 260, 7562-7567. 4. Manjunath, G. et al., Postgrad. Med. 2001, 110, 55-62. 5. Gross, J.L. et al., Diabetes Care, 2005, 28, 164-176. 6. Anavekar, N.S. et al., New Engl. J. Med., 2004, 351, 1285-1295.
• Assay Range: 0.3125- 20 mg/dl
• Description: Quantitative colorimetric detection of creatinine in urine samples
• Sensitivity: 0.032 mg/dl
• Component No: SKC-200A | SKC-200B | SKC-200C | SKC-200D
• Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
• Net Weight G: 300
• Product Type: Detection Kits
• Sample Types: Urine
• Stock Product: Y
• Assay Overview: The Urine Creatinine Detection kit is designed to quantitatively measure creatinine present in urine samples. A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate. The color generating reaction is initiated with the StressXpress® Creatinine Reagent, which is pipetted into each well. After a short incubation the intensity of the generated color is detected in a microtiter plate reader capable of measuring 490nm wavelength. The concentration of the creatine in the sample is calculated, after making a suitable correction for the dilution of the sample, using software available with most plate readers. The Jaffe reaction used in this kit has been modified to read creatinine levels in urine(7,8).
• Quantity Size: 2 Plates | 1 ml | 20 ml | 2 each
• Research Areas: Cell Signaling | Cardiovascular System
• Gross Weight Kg: 2
• Detection Method: Colorimetric Assay
• Alternative Names: N-Carbamimidoyl-N-methylglycine Detection Kit, Methylguanidoacetic acid Detection Kit
• Cite This Product: Urine Creatinine Detection Kit (StressMarq Biosciences, Canada, Cat # SKT-200-192)
• Country of Origin: Canada
• Number of Samples: 88 samples in duplicate
• Storage Temperature: 4ºC
• Shipping Temperature: Blue Ice
• Scientific Background: Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP (1). Creatine either comes from the diet or synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist (2). Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH2. Creatinine forms spontaneously from p-creatine (3). Under normal conditions, its formation occurs at a rate that is relatively constant and as intra-individual variation is <15% from day to day, creatinine is a useful tool for normalizing the levels of other molecules found in urine. Additionally altered creatinine levels may be associated with other conditions that result in decreased renal blood flow such as diabetes and cardiovascular disease (4-6).
• Species Reactivity Medium: Human | Monkey | Dog | Rat
• Species Reactivity Full Name: Human | Monkey | Dog | Rat

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