79760
PDGFRα (D842Y) Assay Kit
BPS Bioscience
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DESCRIPTION
Platelet-derived growth factor receptor A or PDGFRα has been implicated in regulation of cell growth and survival, apoptosis, and differentiation. It has been identified as a potential target in eosinophilic leukemia cancer, inflammatory breast cancer, and gastrointestinal stromal tumors. The D842 mutation is found in gastrointestinal stromal tumors and mutations at this site, other than D842Y, are associated with resistance to tyrosine kinase inhibitors. The PDGFRα (D842Y) Assay Kit is designed to measure PDGFRα (D842Y) activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
DETAILS
- Shiptemp: -80°C (dry ice)
- Synonyms: Platelet Derived Growth Factor Receptor Alpha, CD140 Antigen-Like Family Member A, PDGFR2, CD140a Antigen
- Warnings: Avoid freeze/thaw cycles.
- Category: Kinase/Assay Kit
- References: 1. Evans, E., et al. A Precision Therapy Against Cancers Driven by KIT/PDGFRA Mutations; 2017, Science Translational Medicine Nov; 9(414): 1690. 2. Joglekar-Javadekar, M., et al. Characterization and Targeting of Platelet-Derived Growth Factor Receptor alpha (PDGFRA) in Inflammatory Breast Cancer (IBC). Neoplasia. 2017 Jul; 19(7):564-573. 3. Corless, C.L., et al. PDGFRA mutations in gastrointestinal stromal tumors: frequency, spectrum and in vitro sensitivity to imatinib. J Clin Oncol. 2005 Aug 10; 23(23):5357-64.
- Description: Platelet-derived growth factor receptor A or PDGFRα has been implicated in regulation of cell growth and survival, apoptosis, and differentiation. It has been identified as a potential target in eosinophilic leukemia cancer, inflammatory breast cancer, and gastrointestinal stromal tumors. The D842 mutation is found in gastrointestinal stromal tumors and mutations at this site, other than D842Y, are associated with resistance to tyrosine kinase inhibitors. The PDGFRα (D842Y) Assay Kit is designed to measure PDGFRα (D842Y) activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
- Methodology: Chemiluminescent
- Supplied As: The PDGFRα (D842Y) Assay Kit comes in a convenient 96-well format, with enough purified recombinant PDGFRα (D842Y), Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1), ATP, and kinase assay buffer for 96 enzyme reactions.
- Unspsc Code: 41105332
- Unspsc Name: Protein chemiluminescent detection reagents or kits or substrates
- Applications: Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
- Product Type: Assay Kit
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 40261, 100201, 100202, 79761, 78487
- Storage Stability: Up to 6 months when stored as recommended.
- Scientific Category: Kinase
- Instructions for Use: See assay protocol for detailed instructions.