DESCRIPTION

• Aa: 2-1102 (end)
• Mw: 130 kDa
• Tags: N-terminal His-FLAG-tags
• Format: Aqueous buffer solution
• Genbank: NM_003470
• Species: Human
• Uniprot: Q93009
• Shiptemp: -80°C (dry ice)
• Synonyms: Ubiquitin specific peptidase 7, USP 7, HAUSP, USP-7
• Warnings: Avoid freeze/thaw cycles.
• Category: Ubiquitination/Deubiquitylating Enzymes
• References: 1. Fernandez-Montalvan, A., et al., FEBS J. 2007 Aug;274(16):4256-70.2. Li, M., et al., Mol Cell. 2004 Mar 26;13(6):879-86.
• Description: Human ubiquitin specific peptidase 7 (USP7) or HAUSP, GenBank Accession No. NM_003470, a.a. 2 - 1102 (end) with N-terminal His- Flag tag, MW = 130 kDa, expressed in a baculovirus infected Sf9 cell expression system.
• Formulation: 47.5 mM Tris-HCl, pH 8.0, 131 mM NaCl, 2.6 mM KCl, 90 mM imidazole, 5% glycerol
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Reaction was performed in 50 mM Tris pH 7.4, 1 mM DTT. 0.5 mM EDTA, and 500 nM Ub-AMC. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation of 340 nm, and emission at 460 nm.
• Specific Activity: 46.0 pmole/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Ubiquitins: Deubiquitinase (DUB)