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100692-1 100692-2

FGFR1, Avi-His-Tag, Biotin-labeled (Dephosphorylated) Recombinant

BPS Bioscience

DESCRIPTION

Human Fibroblast growth factor receptor 1 (FGFR1), also known as BFGFR, CD331, CEK and FLT2, GenBank Accession No. NM_0023110.3, a.a. 399-822(end), with C-terminal Avi-His-tag, co-expressed with PTP1B for dephosphorylation in an Sf9 cell expression system. Percentage of phosphorylation ≤5% and enzymatically biotinylated using Avi-tag™ technology. Biotinylation is confirmed to be ≥90%. MW=51 kDa.

DETAILS

  • Aa: 399-822(end)
  • Mw: 51 kDa
  • Tags: C-terminal Avi-His-Tag
  • Format: Aqueous buffer solution
  • Genbank: NM_0023110.3
  • Species: Human
  • Uniprot: P11362
  • Shiptemp: -80°C (dry ice)
  • Synonyms: Human Fibroblast growth factor receptor 1, BFGFR, CD331, CEK, FLT2, FGFR-1
  • Warnings: Avoid freeze/thaw cycles.
  • Category: Kinase/Receptor Tyrosine
  • References: 1. Weiss, J. et al., Sci. Trans. Med. (2010); 2(62):62ra93.2. Dode, C. et al., Nature Genetics. (2003); 33:463-465.
  • Description: Human Fibroblast growth factor receptor 1 (FGFR1), also known as BFGFR, CD331, CEK and FLT2, GenBank Accession No. NM_0023110.3, a.a. 399-822(end), with C-terminal Avi-His-tag, co-expressed with PTP1B for dephosphorylation in an Sf9 cell expression system. Percentage of phosphorylation ≤5% and enzymatically biotinylated using Avi-tag™ technology. Biotinylation is confirmed to be ≥90%. MW=51 kDa.
  • Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.02% Tween, 20% glycerol, 3 mM DTT.
  • Supplied As: Aqueous buffer solution.
  • Unspsc Code: 12352202
  • Unspsc Name: Proteins
  • Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
  • Product Type: Protein
  • Biosafety Level: Not applicable (BSL-1)
  • Assay Conditions: Assay was done according to TYR Phosphorylation Assay Kit (BPS Bioscience #79502) with FGFR enzyme titration range 0 ng/rxn-500 ng/rxn.
  • Related Products: 40210, 40209, 79804, 100613, 78487
  • Storage Stability: At least 6 months at -80°C.
  • Scientific Category: Kinase Recptor Tyrosine