DNMT3B/DNMT3L Complex, His-tag, GST-tag Recombinant

DESCRIPTION

Complex of human DNA methyl-transferase 3B (DNMT3B), GenBank Accession No. NM_006892, a.a. 564-853(end) with N-terminal His-tag, MW=34 kDa, and DNMT3L (GenBank Accession No. NM_013369), a.a. 160-387(end), with an N-terminal GST-tag, MW = 52 kDa, co-expressed in a baculovirus-infected Sf9 cell expression system.

DETAILS

Aa: 564-853(end), DNMT3b; 160-387(end), DNMT3L
Mw: 86 kDa (complex)
Tags: N-terminal His, N-terminal GST-tag
Format: Aqueous buffer solution
Genbank: NM_006892(DNMT3B), NM_013369 (DNMT3L)
Species: Human
Uniprot: Q9UBC3 (DNMT3B), Q9UJW3 (DNMT3L)
Shiptemp: -80°C (dry ice)
Warnings: Avoid freeze/thaw cycles.
Category: Methyltransferase/Enzymes
Description: Complex of human DNA methyl-transferase 3B (DNMT3B), GenBank Accession No. NM_006892, a.a. 564-853(end) with N-terminal His-tag, MW=34 kDa, and DNMT3L (GenBank Accession No. NM_013369), a.a. 160-387(end), with an N-terminal GST-tag, MW = 52 kDa, co-expressed in a baculovirus-infected Sf9 cell expression system.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, and 20% glycerol
Unspsc Code: 12352202
Unspsc Name: Proteins
Applications: Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.
Host Species: insect
Product Type: Proteins
Host Cell Line: Sf9 cells
Biosafety Level: Not applicable (BSL-1)
Assay Conditions: Assayed using BPS Bioscience DNMT3B Assay Kit, Cat. #52034. Add 50 µl reaction mix containing DNMT3B/DNMT3L to wells coated with DNMT substrate. Incubate 2 hr at 37°C. Add antibody against 5-methylcytosine, then HRP-labeled secondary antibody followed by chemiluminescent detection.
Related Products: 52034, 51103, 51105, 51109, 51106
Specific Activity: ≥0.52 pmol/min/µg.
Storage Stability: At least 6 months at -80°C.
Scientific Category: Methyltransferase