H2DCFDA (2',7'-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2',7'-dichlorofluorescein.λEx/λ Em (pH>6) = 504/529 nm (end product)White solid soluble in DMSO or DMFStore desiccated at -20°C and protect from lightC24H16Cl2O7MW: 487.29[4091-99-0]