DESCRIPTION

• Target: NKCC2
• Gene Id: 25065
• Conjugate: ATTO 594
• Immunogen: Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody desgination L224).
• References: 1. Caceres P.S., Ares G.R., and Ortiz P.A. (2009) J Biol Chem. 284(37): 24965-24971. 2. Xue H., et al. (2009) Neurogastroentrol Motil. 21(10): 1068-1089.
• Swiss-prot: Q5PQV1
• Specificity: Detects ~160kDa.
• Applications: WB | IHC
• Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
• Host Species: Rabbit
• Product Type: Polyclonal
• Purification: Protein A Purified
• Concentration: 1 mg/ml
• Research Areas: Cancer | Neuroscience | Pumps/Transporters | Ion Pumps/Transporters
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Accession Number: NP_001257546.1
• Alternative Names: SLC12A1 Antibody, sodium potassium chloride cotransporter 2 Antibody, sodium-potassium-chloride cotransporter isoform 2 Antibody, BSC1 Antibody, MBSC1 Antibody, Na-K-2Cl cotransporter Antibody, NKCC2A variant A Antibody, RBSC Antibody
• Antibody Dilution: WB (1:1000); IHC (1:25); optimal dilutions for assays should be determined by the user.
• Cite This Product: StressMarq Biosciences Cat# SPC-401D-A594, RRID: AB_2704508
• Immunogen Species: Rat
• Storage Temperature: Conjugated antibodies should be stored according to the product label
• Shipping Temperature: Blue Ice or 4ºC
• Cellular Localization: Membrane
• Scientific Background: NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell (1, 2). Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide (1).
• Certificate of Analysis: 1 µg/ml of SPC-401 was sufficient for detection of NKCC2 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Species Reactivity Full Name: Human | Mouse | Rat