DESCRIPTION

• Target: Protocadherin Gamma
• Gene Id: 93709
• Isotype: IgG1
• Conjugate: HRP
• Immunogen: Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941). 99% identity with human (123/124 amino acids).
• Swiss-prot: Q91XZ0
• Specificity: Detects ~100kDa. Cross-reacts with all Gamma-protocadherin-A, -B and -C proteins.
• Applications: WB | IHC | ICC/IF
• Clone Number: N159/5 (Formerly sold as S159-5)
• Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
• Host Species: Mouse
• Product Type: Monoclonal
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Research Areas: Neuroscience | Cell Markers | Neuron Markers | Membrane Markers
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer may change when conjugated
• Accession Number: NP_291062.1
• Alternative Names: PCDH gamma A Antibody, PCDH-gamma-A Antibody, PCDHGA Antibody, Protocadherin gamma A Antibody, Protocadherin gamma subfamily A Antibody, Gamma Protocadherin A (pan) Antibody, Pan-Gamma-Protocadherin-A Antibody, Pan Gamma Protocadherin A Antibody
• Antibody Dilution: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Cite This Product: StressMarq Biosciences Cat# SMC-454D-HRP, RRID: AB_2701932
• Immunogen Species: Mouse
• Storage Temperature: Conjugated antibodies should be stored according to the product label
• Shipping Temperature: Blue Ice or 4ºC
• Cellular Localization: Cell Membrane
• Scientific Background: The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
• Certificate of Analysis: 1 µg/ml of SMC-454 was sufficient for detection of Protocadherin gamma (pan) in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Species Reactivity Full Name: Human | Mouse | Rat