60090
PDE9A2, GST-tag Recombinant
BPS Bioscience
DESCRIPTION
Human PDE9A2 (GenBank Accession No. NM_001001567), full length with N-terminal GST-tag, MW=87.7 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
DETAILS
- Aa: full length
- Mw: 87.7 kDa
- Tags: N-terminal GST-tag
- Format: Aqueous buffer solution
- Genbank: NM_001001567
- Species: Human
- Uniprot: O76083
- Shiptemp: -80°C (dry ice)
- Synonyms: PDE9A, phosphodiesterase 9A
- Warnings: Avoid freeze/thaw cycles.
- Category: Phosphodiesterase/Enzymes
- References: 1. Rentero C. and Puigdomenech P., BMC Mol. Biol. 7, 39 (2006).2. Huai, Q., et al., Journal Proc. Natl. Acad. Sci. U.S.A. 101 (26), 9624-9629 (2004). Application Reference(s): 1. Characterization of the binding properties of T-773 as a PET radioligand for phosphodiesterase 10A (2015) 2. Active Site Coupling in PDE:PKA Complexes Promotes Resetting of Mammalian cAMP Signaling (2014) 3. Genetic deletion and pharmacological inhibition of phosphodiesterase 10A protects mice from diet-induced obesity and insulin resistance (2014) 4. A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition (2013) 5. Analysis of substrate specificity and kinetics of cyclic nucleotide phosphodiesterases with N'-methylanthraniloyl-substituted purine and pyrimidine 3',5'-cyclic nucleotides by fluorescence spectrometry (2013) 6. The novel phosphodiesterase 10A inhibitor THPP-1 has antipsychotic-like effects in rat and improves cognition in rat and rhesus monkey (2013)
- Description: Human PDE9A2 (GenBank Accession No. NM_001001567), full length with N-terminal GST-tag, MW=87.7 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
- Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 3 mM DTT and 20% glycerol
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
- Host Species: insect
- Product Type: Protein
- Host Cell Line: Sf9 cells
- Biosafety Level: Not applicable (BSL-1)
- Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.
- Assay Conditions: Assayed using BPS's PDE9A kit, Cat. # 60390. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 200 µM cGMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE9A2 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the_x000D_ 5' -GMP product and releasing the_x000D_ phosphate group which is detected by_x000D_ Malachite Green Reagent.
- Specific Activity: ≥200 pmol/min/µg.
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: Phosphodiesterase