Anti-CD244/2B4/SLAMF4 (Extracellular region) M053 Antibody

DETAILS

Form: Protein G Purified
Gene: CD244
Host: Mouse
Rrid: AB_3068389
Type: Primary Antibody
Clone: M053
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: CD244/2B4/SLAMF4 (Extracellular region) M053 Antibody
Isotype: IgG1
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: Natural killer cell receptor 2B4, 2B4, NK cell activation-inducing ligand, NAIL NK cell type I receptor protein 2B4, NKR2B4, h2B4, SLAM family member 4, SLAMF4, Signaling lymphocytic activation molecule 4, CD244
Clonality: Mouse Monoclonal
Immunogen: Clone (M053) was generated from a recombinant protein that included the extracellular region of human CD244 protein.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: Clone M053 mouse monoclonal antibody detects a 110 kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human THP1 monocytes. The antibody prefers the native CD244 protein, but also detects denatured CD244. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.
Applications: WB, IP, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 110
Dilution Range Ip: 1:100
Dilution Range Wb: 1:500
Immunogen Species: Human
Dilution Range Icc: 1:200
Species Reactivity: Human
Target Description: CD244 (Natural killer (NK) cell receptor 2B4/SLAMF4) is an Ig superfamily signaling lymphocyte activation molecule (SLAM) receptor. Like all SLAM family receptors, it has an extracellular segment with two immunoglobulin (Ig)-like domains, and a cytoplasmic domain containing four immunoreceptor tyrosine-based switch motifs. CD244 does not act as a selfligand similar to other SLAM family receptors. It binds CD48, a transmembrane receptor ubiquitously expressed on hematopoietic cells. CD244 activity is controlled by the presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Downstream signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Activation of CD244 stimulates NK cell cytotoxicity, production of IFN-γ and granule exocytosis. CD244 is involved in the regulation of CD8+ T-cell proliferation, and inhibits inflammatory responses in dendritic cells (DCs). In cancers, CD244 shows increased expression in intratumoral DCs and myeloid suppressor cells, and anti-CD244 therapies may increase infiltrating T-cells and impair tumor growth.
Uniprot Number Immunogen Species: Q9BZW8
Post Translational Modification Type: GOmAb