RG240055
STAG3 (NM_001282716) Human Tagged ORF Clone
Origene Technologies, Inc.
DESCRIPTION
STAG3 (tGFP-tagged) - Human stromal antigen 3 (STAG3), transcript variant 2
DETAILS
- Mw: 139 kDa
- Tag: TurboGFP
- Accn: NM_001282716
- Refseq: NM_001282716.1, NP_001269645.1
- Status: 2 Weeks*
- Symbol: STAG3
- Vector: pCMV6-AC-GFP
- Category: cDNA Clones
- Locus Id: 10734
- Orf Size: 3675 bp
- Refseq Orf: 3678 bp
- Uniprot Id: Q9UJ98
- Refseq Size: 4313 bp
- Availability: 2 Weeks
- Cytogenetics: 7q22.1
- Gene Summary: The protein encoded by this gene is expressed in the nucleus and is a subunit of the cohesin complex which regulates the cohesion of sister chromatids during cell division. A mutation in this gene is associated with premature ovarian failure. Alternate splicing results in multiple transcript variants encoding distinct isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Apr 2014]
- Sequence Data: ORF Nucleotide Sequence ( show hide ) >RG240055 representing NM_001282716. Blue =ORF Red =Cloning site Green =Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGC C ATGTCTTCCCCGTTGCAAAGAGCTGTGGGAGATACCAAGAGGGCCTTGTCTGCATCTTCTAGTTCCTCT GCCAGTCTACCCTTTGATGACAGGGACTCAAACCATACCTCAGAGGGGAATGGCGACTCTTTGTTAGCT GATGAAGACACTGACTTTGAAGACAGCTTGAATCGCAATGTGAAGAAGAGAGCAGCAAAACGACCACCG AAAACAACACCGGTGGCAAAACATCCAAAGAAAGGGTCCCGAGTGGTACATCGTCATAGCCGGAAACAG TCAGAGCCACCAGCCAATGATCTTTTCAATGCTGTGAAAGCCGCCAAAAGTGACATGCAGTCTTTGGTA GATGAGTGGCTGGATAGCTACAAGCAAGACCAGGATGCAGGATTTCTGGAGCTTGTTAACTTTTTCATC CAATCTTGCGGATGTAAAGGCATTGTGACCCCTGAGATGTTCAAGAAGATGTCCAACTCAGAGATCATC CAGCACCTAACAGAGCAGTTTAATGAGGACTCGGGGGACTACCCTCTCATAGCTCCAGGTCCATCCTGG AAGAAGTTCCAGGGCAGCTTCTGTGAATTTGTGAGGACATTGGTCTGTCAGTGCCAGTACAGCCTCCTC TATGATGGCTTCCCTATGGACGACCTCATCTCCCTGCTCACTGGCCTCTCAGACTCACAAGTCCGCGCC TTCCGTCACACTAGCACCCTGGCTGCTATGAAACTGATGACCTCCCTGGTAAAAGTTGCCCTCCAACTG AGTGTGCACCAAGATAACAATCAGCGTCAGTATGAGGCTGAAAGAAACAAGGGGCCAGGGCAGAGGGCA CCTGAGCGGCTGGAGAGCCTGTTGGAGAAACGCAAAGAGCTCCAAGAGCATCAAGAGGAGATTGAGGGG ATGATGAATGCCCTCTTCAGGGGTGTCTTTGTTCATCGGTACAGGGATGTCCTTCCTGAGATCCGTGCT ATCTGCATTGAGGAAATTGGGTGTTGGATGCAAAGCTACAGCACGTCTTTCCTCACCGACAGCTATTTA AAATATATTGGTTGGACTCTGCATGATAAGCACCGAGAAGTCCGCCTGAAGTGTGTGAAGGCCCTGAAA GGGCTGTACGGTAACCGGGACCTGACCACACGCCTGGAGCTCTTCACCAGCCGCTTCAAGGACCGGATG GTTTCCATGGTCATGGACAGAGAGTATGATGTGGCAGTGGAGGCTGTCAGATTACTGATACTTATCCTT AAGAACATGGAAGGGGTGCTGACGGACGCGGATTGTGAGAGCGTCTACCCAGTTGTGTATGCCTCTCAT CGAGGCCTGGCCTCTGCCGCAGGCGAATTTCTGTACTGGAAACTCTTCTACCCTGAGTGCGAGATAAGA ATGATGGGTGGAAGAGAGCAACGCCAGAGCCCAGGCGCCCAGAGGACTTTCTTCCAGCTTCTGCTGTCC TTCTTTGTGGAGAGCGAGCTCCATGACCACGCTGCTTACTTAGTAGACAGTCTGTGGGACTGTGCAGGG GCTCGGCTGAAGGACTGGGAGGGTCTGACAAGCCTGCTGCTGGAGAAGGACCAGAACCTGGGTGATGTG CAGGAGAGCACACTGATAGAAATCCTTGTGTCCAGTGCCCGGCAAGCTTCAGAGGGGCACCCGCCTGTG GGCCGGGTCACTGGGAGGAAGGGCTTAACCTCTAAGGAGCGCAAGACCCAAGCCGATGACAGGGTGAAG TTGACTGAGCACCTCATCCCCCTGCTGCCCCAGCTCCTGGCCAAGTTCTCAGCTGATGCAGAGAAGGTC ACTCCCCTGCTCCAGCTTCTCAGCTGCTTTGACCTCCACATCTACTGCACTGGGCGCTTGGAGAAGCAC CTGGAGCTGTTCCTGCAGCAACTCCAGGAGGTGGTGGTGAAGCATGCAGAGCCAGCGGTGCTTGAGGCT GGGGCGCATGCCCTCTACCTGCTCTGTAATCCCGAATTCACTTTCTTCAGCCGGGCGGACTTTGCCCGC AGCCAGCTAGTAGATTTGCTGACTGACCGCTTCCAGCAGGAGCTTGAAGAGCTGTTACAGTCGTCCTTC CTAGATGAGGATGAGGTATATAATCTGGCAGCCACTCTGAAACGCCTCTCTGCCTTCTACAACACTCAT GACCTGACTCGCTGGGAGCTCTATGAGCCATGTTGCCAACTCCTGCAGAAGGCTGTGGACACAGGAGAG GTTCCTCACCAGGTTATCCTGCCAGCCTTGACTCTTGTCTATTTTTCCATTCTCTGGACACTAACCCAC ATTTCTAAATCAGATGCTTCCCAGAAGCAGCTGTCGAGTTTGAGGGACAGAATGGTGGCCTTCTGTGAA CTCTGCCAGAGTTGCCTCTCAGATGTGGATACTGAGATCCAGGAGCAGGCTTTTGTCTTATTAAGTGAT CTACTTCTCATCTTTAGCCCTCAGATGATTGTTGGGGGCCGTGATTTCCTTAGGCCACTTGTCTTTTTT CCTGAAGCTACTCTCCAGTCTGAGCTAGCCAGCTTCCTCATGGACCACGTCTTCATCCAGCCGGGAGAC CTGGGCAGTGGTGATTCCCAGGAGGATCATTTACAGATAGAGCGGCTACACCAGCGGCGCCGCCTCCTA GCCGGGTTCTGCAAGCTGTTGCTTTATGGGGTGCTGGAGATGGATGCAGCCTCAGATGTTTTCAAACAC TACAACAAGTTCTACAATGACTATGGTGACATTATCAAGGAAACATTAACTAGAGCAAGGCAGATTGAC CGAAGTCATTGTTCCCGAATCCTGCTGCTGAGCCTCAAGCAGCTGTACACAGAACTGCTGCAGGAGCAT GGGCCCCAGGGCCTGAATGAGCTTCCTGCCTTCATCGAGATGAGGGACCTGGCCCGGAGGTTTGCCTTG AGTTTTGGACCCCAGCAGCTGCAGAACCGTGACCTCGTGGTCATGCTACACAAGGAAGGCATCCAGTTC TCCTTGTCTGAGCTTCCTCCAGCTGGCTCCTCCAATCAGCCTCCAAATCTGGCATTCCTGGAGCTCCTT TCAGAGTTTTCCCCCCGACTCTTCCATCAGGACAAGCAGCTTTTACTGTCCTATCTAGAAAAGTGCCTG CAGCATGTCTCCCAGGCACCTGGCCATCCCTGGGGCCCAGTCACCACCTACTGCCACTCCCTCAGCCCT GTGGAGAACACAGCAGAGACCAGCCCTCAGGTCCTCCCCAGCTCCAAGAGGAGGCGCGTTGAAGGGCCT GCCAAGCCTAACAGAGAGGACGTCTCCTCGTCCCAGGAAGAAAGTCTGCAGCTGAACAGCATCCCGCCC ACGCCCACCCTCACCTCCACAGCTGTGAAGAGCAGGCAGCCCCTGTGGGGGTTGAAAGAGATGGAGGAA GAAGATGGCTCAGAGTTGGATTTTGCCCAGGGTCAGCCCGTCGCAGGCACCGAGAGGTCAAGGTTCTTG GGTCCACAATATTTCCAGACTCCACACAACCCTTCAGGTCCTGGCCTGGGCAACCAGCTGATGCGACTC AGCCTTATGGAAGAGGACGAGGAAGAAGAGTTAGAAATCCAGGATGAGTCAAATGAAGAACGGCAGGAT ACAGACATGCAAGCAAGTAGCTACTCTTCCACCAGTGAGCGCGGGCTGGACCTCTTAGATTCTACAGAG CTGGATATTGAGGATTTC ACGCGT ACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC Protein Sequence ( show hide ) >Peptide sequence encoded by RG240055 Blue =ORF Red =Cloning site Green =Tag(s) MSSPLQRAVGDTKRALSASSSSSASLPFDDRDSNHTSEGNGDSLLADEDTDFEDSLNRNVKKRAAKRPP KTTPVAKHPKKGSRVVHRHSRKQSEPPANDLFNAVKAAKSDMQSLVDEWLDSYKQDQDAGFLELVNFFI QSCGCKGIVTPEMFKKMSNSEIIQHLTEQFNEDSGDYPLIAPGPSWKKFQGSFCEFVRTLVCQCQYSLL YDGFPMDDLISLLTGLSDSQVRAFRHTSTLAAMKLMTSLVKVALQLSVHQDNNQRQYEAERNKGPGQRA PERLESLLEKRKELQEHQEEIEGMMNALFRGVFVHRYRDVLPEIRAICIEEIGCWMQSYSTSFLTDSYL KYIGWTLHDKHREVRLKCVKALKGLYGNRDLTTRLELFTSRFKDRMVSMVMDREYDVAVEAVRLLILIL KNMEGVLTDADCESVYPVVYASHRGLASAAGEFLYWKLFYPECEIRMMGGREQRQSPGAQRTFFQLLLS FFVESELHDHAAYLVDSLWDCAGARLKDWEGLTSLLLEKDQNLGDVQESTLIEILVSSARQASEGHPPV GRVTGRKGLTSKERKTQADDRVKLTEHLIPLLPQLLAKFSADAEKVTPLLQLLSCFDLHIYCTGRLEKH LELFLQQLQEVVVKHAEPAVLEAGAHALYLLCNPEFTFFSRADFARSQLVDLLTDRFQQELEELLQSSF LDEDEVYNLAATLKRLSAFYNTHDLTRWELYEPCCQLLQKAVDTGEVPHQVILPALTLVYFSILWTLTH ISKSDASQKQLSSLRDRMVAFCELCQSCLSDVDTEIQEQAFVLLSDLLLIFSPQMIVGGRDFLRPLVFF PEATLQSELASFLMDHVFIQPGDLGSGDSQEDHLQIERLHQRRRLLAGFCKLLLYGVLEMDAASDVFKH YNKFYNDYGDIIKETLTRARQIDRSHCSRILLLSLKQLYTELLQEHGPQGLNELPAFIEMRDLARRFAL SFGPQQLQNRDLVVMLHKEGIQFSLSELPPAGSSNQPPNLAFLELLSEFSPRLFHQDKQLLLSYLEKCL QHVSQAPGHPWGPVTTYCHSLSPVENTAETSPQVLPSSKRRRVEGPAKPNREDVSSSQEESLQLNSIPP TPTLTSTAVKSRQPLWGLKEMEEEDGSELDFAQGQPVAGTERSRFLGPQYFQTPHNPSGPGLGNQLMRL SLMEEDEEEELEIQDESNEERQDTDMQASSYSSTSERGLDLLDSTELDIEDF TR TRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
- Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
- Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
- E Coli Selection: Ampicillin (100 ug/mL)
- Protein Pathways: Oocyte meiosis
- Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
- Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
- Mammalian Cell Selection: Neomycin