DESCRIPTION

• Target: GRP170
• Gene Id: 10525
• Isotype: IgG2b
• Conjugate: ATTO 594
• Immunogen: Recombinant Full length Human GRP170 Protein
• References: 1. Wang H., et al. (2014) Front Oncol. 4: 377. 2. Manjili M.H., et al. (2006) Immun. Cell Biol. 84: 203-208.
• Swiss-prot: Q9Y4L1
• Specificity: Detects ~170kDa.
• Applications: WB | ICC/IF
• Clone Number: 6E3-2C3
• Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
• Host Species: Mouse
• Product Type: Monoclonal
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Research Areas: Cancer | Heat Shock | Neuroscience | Neurodegeneration | Epigenetics and Nuclear Signaling | Cardiovascular System |Cell Signaling | Protein Trafficking | Chaperone Proteins | ER Proteins | Cancer | Invasion/microenvironment | Cancer | Cancer Metabolism | Response to Hypoxia | Cell Signaling | Metabolism | Metabolism processes | Hypoxia
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.1% sodium azide *Storage buffer may change when conjugated
• Accession Number: NP_006389.3
• Alternative Names: ORP150 Antibody, HSP12A Antibody, Hypoxia up regulated 1 Antibody, Orp150 Antibody, Glucose regulated Antibody 170 Antibody, 150kDa oxygen regulated Antibody Antibody, 150 kDa oxygen regulated protein antibody, 150 kDa oxygen-regulated protein antibody, 170 kDa glucose regulated protein antibody, 170 kDa glucose-regulated protein antibody, DKFZp686N08236 antibody, FLJ94899 antibody, FLJ97572 antibody, glucose regulated protein 170 antibody, Grp 170 antibody, GRP-170 antibody, Grp170 antibody, HSP 12A antibody, HSP12A antibody, Hyou1 antibody, HYOU1_HUMAN antibody, Hypoxia up regulated 1 antibody, hypoxia up-regulated 1 precursor antibody, Hypoxia up-regulated protein 1 antibody, Hypoxia upregulated 1 antibody, Orp 150 antibody, ORP-150 antibody, Orp150 antibody, oxygen regulated protein (150kD) antibody
• Antibody Dilution: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Cite This Product: StressMarq Biosciences Cat# SMC-232D-A594, RRID: AB_2699470
• Immunogen Species: Human
• Storage Temperature: Conjugated antibodies should be stored according to the product label
• Shipping Temperature: Blue Ice or 4ºC
• Cellular Localization: Endoplasmic Reticulum | Endoplasmic Reticulum Lumen
• Scientific Background: GRP170, also known as ORP150, is the largest member of glucose-regulated Antibodys, and acts as a human chaperone Antibody. It is thought to play an important role in Antibody folding and secretion in the ER. Suppression of the Antibody is associated with accelerated apoptosis, therefore having an important cryoprotective role in hypoxia-induced cellular pertubation. This cryopotective role has led to an anti-tumor immune response, which will hopefully lead to therapeutic immunizations against cancers (1). GRP170 has also been shown to bind with dendritic cells and provide the danger signals to induce anti-tumor immune responses (2).
• Certificate of Analysis: 1 µg/ml of SMC-232 was sufficient for detection of GRP170 in 20 µg of HEK293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Species Reactivity Full Name: Human | Mouse | Rat