DESCRIPTION

• Notes: The AAV-DJ viruses are covered under several patents, including U.S. Patent Nos. 7,588,772, 8,067,014, 8,574,583, and 8,906,387, as well as corresponding foreign patents applications and patent rights. AAV-DJ is used under a license agreement. Biosafety: Recombinant AAV is inherently replication-deficient and not known to cause any human diseases. Additionally, following transduction, AAV vectors exist episomally and do not integrate into or disrupt the host cell's genome. AAV requires the use of a Biosafety Level 1 facility. BPS Bioscience recommends following all local, federal, state, and institutional regulations and using all appropriate safety precautions. Troubleshooting Guide: Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com.
• Purity: The purity of the AAV particles was confirmed to be greater than 90% by staining with One-Step Lumitein™ UV Protein Gel Stain (Biotium, 21005-1L). Purity varies with each lot; the exact value will be provided with each shipment.
• Species: AAV Serotype DJ
• Shiptemp: -80°C (dry ice)
• Warnings: Avoid freeze/thaw cycles
• Category: Gene Therapy/AAV
• Background: RNA interference (RNAi) is a highly specific tool for protein knockdown in which a small non-coding RNA binds to the target protein's mRNA to promote its degradation and reduce protein translation. AAV vector-based expression of RNAi drives prolonged reduction in the level of mRNA targets in live animals. Short-hairpin RNA is one of the most widely used RNA interference methods to inhibit protein translation. Adeno-Associated Virus-DJ (AAV-DJ) is a synthetic serotype made from eight different wild-type AAV serotypes (AAV2, 4, 5, 8, 9, avian, bovine, and goat AAV) using DNA shuffling. These modifications allow the AAV-DJ serotype to exhibit improved transduction efficiency in vitro and in vivo and infect a broader range of cell types compared to wild-type serotypes.
• Description: This AAV-DJ Scrambled shRNA (short-hairpin RNA) Control co-expresses a 29-mer scrambled shRNA sequence under the control of a U6 promoter, and Turbo GFP under the control of a CMV promoter to assess the efficacy of transduction.
• Formulation: AAV was produced in HEK293-AAV cells and is supplied in PBS-MK (PBS Magnesium-Potassium) buffer containing 0.01% Pluronic F68. Virus particles can be packaged in custom formulations by special request, for an additional fee.
• Supplied As: Two vials (50 µl x 2) of AAV at a titer ≥1 x 1012 vector genomes/ml. The titer is determined by qPCR and varies with each lot; the exact value will be provided with each shipment.
• Unspsc Code: 41106621
• Unspsc Name: Virus mediated expression vectors or kits
• Applications: Use as a negative control for gene-specific shRNA transduction via AAV vectors.
• Product Type: AAV virus
• Biosafety Level: BSL-1
• Related Products: 78444, 78447, 78463
• Storage Stability: AAV is shipped with dry ice. For long-term storage, it is recommended to store AAV at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
• Scientific Category: Gene & Cell Therapy
• Signed License Required: No