DESCRIPTION

• Tag: Tag Free
• Accn: NM_001048142
• Type: Mouse Untagged Clone
• Refseq: BC034862, AAH34862
• Status: In Stock*
• Symbol: Bdnf
• Vector: PCMV6-Kan/Neo
• Category: cDNA Clones
• Locus Id: 12064
• Refseq Orf: 750 bp
• Uniprot Id: P21237
• Insert Size: 750 bp
• Refseq Size: 1158 bp
• Availability: In Stock
• Cytogenetics: 2 56.63 cM
• Gene Summary: The protein encoded by this gene is a member of the nerve growth factor family. It is involved in the growth, differentiation and survival of specific types of developing neurons both in the central nervous system (CNS) and the peripheral nervous system. It is also involved in regulating synaptic plasticity in the CNS. Expression of a similar gene in human is reduced in both Alzheimer's and Huntington disease patients. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Oct 2015] Transcript Variant: This variant (4) differs in the 5' UTR and coding region, and uses a downstream translation start compared to variant 1. The resulting protein (isoform 2) has a shorter N-terminus compared to isoform 1. Variants 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and 12 encode the same protein.
• Sequence Data: Fully Sequenced ORF ( show hide ) >BC034862 sequence for NM_001048142 CCTGAGTTCCACCAGGTGAGAAGAGTGATGACCATCCTTTTCCTTACTATGGTTATTTCATACTTCGGTT GCATGAAGGCGGCGCCCATGAAAGAAGTAAACGTCCACGGACAAGGCAACTTGGCCTACCCAGGTGTGCG GACCCATGGGACTCTGGAGAGCGTGAATGGGCCCAGGGCAGGTTCGAGAGGTCTGACGACGACATCACTG GCTGACACTTTTGAGCACGTCATCGAAGAGCTGCTGGATGAGGACCAGAAGGTTCGGCCCAACGAAGAAA ACCATAAGGACGCGGACTTGTACACTTCCCGGGTGATGCTCAGCAGTCAAGTGCCTTTGGAGCCTCCTCT ACTCTTTCTGCTGGAGGAATACAAAAATTACCTGGATGCCGCAAACATGTCTATGAGGGTTCGGCGCCAC TCCGACCCTGCCCGCCGTGGGGAGCTGAGCGTGTGTGACAGTATTAGCGAGTGGGTCACAGCGGCAGATA AAAAGACTGCAGTGGACATGTCTGGCGGGACGGTCACAGTCCTAGAGAAAGTCCCGGTATCCAAAGGCCA ACTGAAGCAGTATTTCTACGAGACCAAGTGTAATCCCATGGGTTACACCAAGGAAGGCTGCAGGGGCATA GACAAAAGGCACTGGAACTCGCAATGCCGAACTACCCAATCGTATGTTCGGGCCCTTACTATGGATAGCA AAAAGAGAATTGGCTGGCGATTCATAAGGATAGACACTTCCTGTGTATGTACACTGACCATTAAAAGGGG AAGATAGTGGATTTATGTTGTATAGATTATATTGAGACAAAATTATCTATTTGTATATATACATAACAGG GTAAATTATTCAGTTAAGAAAAAATAATTTTATGAACTGCATGTATAAATGAAGTTTATACAGTACAGTG GTTCTACAATCTATTTATTGGACATATCCATGACCTGAAAGGAAACAGTCATTTGCGCACAACTTTAAAA GTCTGCATTACATTCCTCGATAATGTTGTGGTTTGTTGCCGTTGCCAAGAATTGAAAACAAAAAGTTTAA AAAAAATAATAATAAATTGCATGCTGCTTTAATTGTGAATTGATAATAAACTGTCCCTCTTTCAGAAAAC AGATTAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAA
• Oti Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: EcoRI-NotI     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin