Monoclonal Antibody to Insulin / IRDN (beta-Cell & Insulinoma Marker)(E2-E3 + 2D11-H5; same as INS04 + INS05)

DESCRIPTION

Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

DETAILS

Gene: INS
App Id: IHC
Antigen: Insulin / IRDN (beta-Cell & Insulinoma Marker)
Gene Id: 3630
Isotype: Mouse IgG1, kappa + Mouse IgG1, kappa
Format Id: Purified
Clone Name: E2-E3 + 2D11-H5; same as INS04 + INS05
Uniprot Id: P01308
Host Animal: Mouse
Clonality Id: Monoclonal
Purification: Affinity Chromatography
Reactivity Id: Human, Mouse
Product Content: 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Alternative Name: INS
Application Note: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes);
Storage Condition: Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Immunogen Information: Purified pig insulin, conjµgated to KLH (E2-E3 & 2D11-H5)