Anti-VASP (C-terminal region) Antibody

DETAILS

Form: Protein A Purified
Gene: VASP
Host: Mouse
Rrid: AB_2213406
Type: Primary Antibody
Clone: M277
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: VASP (C-terminal region)
Isotype: IgG1
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: vasodilator-stimulated phosphoprotein
Clonality: Mouse Monoclonal
Immunogen: Clone (M277) was generated from a recombinant protein that includes amino acids from the C-terminal region of human VASP.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody detects 46 and 50 kDa* proteins corresponding to the apparent molecular mass of VASP on SDS-PAGE immunoblots of human A431, HeLa, and HUVEC.
Applications: WB, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 46/50
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:50
Species Reactivity: Human
Target Description: Actin filament tethering and bundling are important mechanisms involved in actin superstructure assembly. The ENA/VASP family includes VASP, mena, and Ena-Vasp-like (EVL). These multidomain proteins localize to the leading edge of filopodia where they associate with AFs, interact with profilin, and compete with capping proteins at the barbed end of AFs. Artificial relocalization of VASP from the plasma membrane to mitochondrial membranes inhibits filopodial formation and axon branching, while deletion of all three ENA/VASP proteins produces defects in cortical axon-tract formation. Regulation of VASP protein activity occurs through phosphorylation at Ser-157, Ser-239, and Thr-278. AMPK phosphorylates Thr-278, leading to impaired actin stress fiber assembly and changes in cell morphology.
Uniprot Number Immunogen Species: P50552
Post Translational Modification Type: GOmAb