DESCRIPTION

• Target: NrCAM
• Gene Id: 319504
• Isotype: IgG2a
• Conjugate: PerCP
• Immunogen: Fusion protein amino acids 30-845 (extracellular domain) of mouse NrCAM. Rat: 96% identity (795/822 amino acids identical). Human: 91% identity (753/822 amino acids identical) ~50% identity with Neurofascin.
• Swiss-prot: Q810U4
• Specificity: Detects ~160kDa.
• Applications: WB | ICC/IF
• Clone Number: N364/51 (Formerly sold as S364-51)
• Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
• Host Species: Mouse
• Product Type: Monoclonal
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Research Areas: Neuroscience | Cell Markers | Neuron Markers | Membrane Markers
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer may change when conjugated
• Accession Number: NP_001139503.1
• Alternative Names: Neuronal cell adhesion molecule Antibody, Bravo Antibody, hBravo Antibody, KIAA0343 Antibody, MGC138845 Antibody, MGC138846 Antibody, Neuronal surface protein Bravo Antibody, Ng CAM related Antibody, Ng-CAM-related Antibody, NgCAM related cell adhesion molecule Antibody, NgCAM-related cell adhesion molecule Antibody, Nr CAM Antibody, Nr-CAM Antibody
• Antibody Dilution: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Cite This Product: StressMarq Biosciences Cat# SMC-462D-PCP, RRID: AB_2702042
• Immunogen Species: Mouse
• Storage Temperature: Conjugated antibodies should be stored according to the product label
• Shipping Temperature: Blue Ice or 4ºC
• Cellular Localization: Cell Membrane
• Scientific Background: Neuronal cell adhesion molecule (NrCAM) is a cell surface protein of the immunoglobulin (Ig) superfamily. NrCAM (also known as Bravo) contains six Ig domains, five fibronectin repeats, a transmembrane region and an intracellular domain. NrCAM is expressed in brain, spinal cord, peripheral nervous system and pancreas. In the spinal cord, NrCAM acts as a ligand for axonin-1 to guide commissural axons across the floor plate. NrCAM also acts as a ligand for F3 to control actin-dependent growth cone motility. NrCAM interacts with neurofascin and may facilitate the clustering of the cystoskeletal protein ankyrin G and the voltage-dependent sodium channel proteins at the node of Ranvier. NrCAM expression may play a role in the severity of certain types of tumors. NrCAM is overexpressed in high-grade astrocytomas, gliomas and glioblastoma tumor tissues. In the pancreas, NrCAM expression is upregulated in intraductal hyperplasia. Antisense NrCAM reduces the tumorigenic properties of human glioblastoma cells in vitro and slowed tumor growth in vivo. The gene encoding human NrCAM maps to chromosome 7q31.1-q31.2.
• Certificate of Analysis: 1 µg/ml of SMC-462 was sufficient for detection of NrCAM in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Species Reactivity Full Name: Human | Mouse | Rat