DESCRIPTION

• Mw: 66.8 kDa
• Tag: Myc-DDK
• Accn: NM_001146204
• Type: Human Tagged ORF Clone
• Refseq: NM_001146204.2
• Status: In Stock*
• Symbol: RASGRP 4
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 115727
• Orf Size: 1812 bp
• Refseq Orf: 1815 bp
• Uniprot Id: Q8TDF6
• Availability: In Stock
• Cytogenetics: 19q13.2
• Gene Summary: The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]
• Chromatograms: CHROMATOGRAMS Sequencher program is needed, download here.
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC228493 representing NM_001146204 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGAACAGAAAAGACAGTAAGAGGAAGTCCCACCAGGAATGCACCGGAAAAATAGGAGGGCGAGGCCGGC CCCGCCAAGTGCGCCGCCACAAGACATGCCCCAGCCCTCGGGAAATCAGCAAGGTCATGGCTTCCATGAA CCTGGGCCTGCTGAGTGAGGGCGGCTGCAGCGAAGATGAGCTGCTGGAGAAATGCATCCAGTCCTTCGAT TCAGCTGGCAGCCTGTGCCACGAGGACCACATGCTCAACATGGTGCTGGCCATGCACAGCTGGGTGCTGC CGTCCGCCGACCTGGCTGCCCGCCTGCTGACCTCATACCAGAAGGCCACAGGGGACACCCAGGAGCTGAG ACGGCTGCAGATCTGTCACCTGGTCAGGTACTGGCTGATGCGACACCCTGAGGTGATGCACCAGGATCCC CAGCTAGAAGAAGTCATAGGTCGTTTCTGGGCCACCGTGGCCCGGGAGGGCAACTCAGCCCAGAGAAGAC TGGGAGACTCTTCTGACCTCCTGAGCCCTGGTGGCCCTGGCCCCCCACTCCCAATGAGCAGCCCAGGCCT GGGCAAAAAGCGCAAAGTGTCCTTGCTTTTCGACCACTTGGAGACGGGGGAGCTGGCTCAGCACCTCACC TACCTGGAGTTCCGGTCCTTCCAGGCTATCACGCCCCAGGACCTGCGGAGCTACGTTTTGCAGGGCTCAG TACGAGGCTGCCCGGCCCTGGAGGGCTCCGTAGGTCTCAGCAACAGCGTGTCCCGCTGGGTGCAGGTGAT GGTGCTGAGCCGTCCCGGGCCCCTACAGCGTGCACAGGTGCTGGACAAGTTCATTCACGTGGCACAGAGG CTCCACCAGCTGCAGAATTTCAACACGCTGATGGCAGTCACAGGGGGCCTGTGTCACAGTGCCATCTCCA GACTCAAGGACTCCCATGCCCACCTGAGCCCTGACAGCACCAAGGAGCTGGTGGCCCTCCAAGGGCAGCA TCCACCCTGCAGCGCCAATGAGGATCTGCTGCACCTGCTCACGCTCTCCCTGGACCTCTTCTACACGGAA GACGAGATCTATGAGCTTTCTTATGCCCGGGAGCCGCGTTGTCCCAAGAGCCTGCCACCCTCCCCCTTCA ATGCACCTCTGGTGGTGGAGTGGGCCCCTGGTGTGACACCCAAGCCGGACAGGGTCACACTGGGTCGGCA TGTGGAGCAGCTGGTGGAGTCTGTGTTCAAGAATTATGACCCTGAAGGCCGAGGAACAATCTCTCAGGAG GACTTTGAGCGACTCTCGGGCAATTTTCCCTTCGCCTGCCATGGGCTTCACCCACCCCCACGCCAGGGGA GAGGATCCTTCAGCAGAGAGGAGCTGACAGGGTACCTGCTCCGGGCCAGCGCCATCTGCTCCAAGTTGGG CCTGGCCTTCCTGCACACCTTCCATGAGGTCACCTTCCGAAAGCCTACCTTCTGCGACAGCTGCAGTGGC TTCCTCTGGGGTGTCACCAAGCAAGGCTACCGCTGTCGGGAGTGCGGGCTGTGTTGCCACAAACACTGCA GAGACCAGGTGAAGGTAGAATGTAAGAAGAGGCCAGGGGCCAAGGGCGATGCAGGACCCCCCGGAGCTCC TGTCCCATCCACACCAGCTCCCCATGCCAGCTGTGGCTCCGAGGAAAATCACTCCTACACGCTATCCCTG GAGCCTGAGACTGGGTGCCAGCTTCGCCATGCCTGGACCCAGACTGAATCCCCACACCCTTCCTGGGAAA CAGATACGGTCCCCTGCCCGGTGATGGACCCACCATCAACTGCATCCTCCAAGCTGGATTCC ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC228493 representing NM_001146204 Red =Cloning site Green =Tags(s) MNRKDSKRKSHQECTGKIGGRGRPRQVRRHKTCPSPREISKVMASMNLGLLSEGGCSEDELLEKCIQSFD SAGSLCHEDHMLNMVLAMHSWVLPSADLAARLLTSYQKATGDTQELRRLQICHLVRYWLMRHPEVMHQDP QLEEVIGRFWATVAREGNSAQRRLGDSSDLLSPGGPGPPLPMSSPGLGKKRKVSLLFDHLETGELAQHLT YLEFRSFQAITPQDLRSYVLQGSVRGCPALEGSVGLSNSVSRWVQVMVLSRPGPLQRAQVLDKFIHVAQR LHQLQNFNTLMAVTGGLCHSAISRLKDSHAHLSPDSTKELVALQGQHPPCSANEDLLHLLTLSLDLFYTE DEIYELSYAREPRCPKSLPPSPFNAPLVVEWAPGVTPKPDRVTLGRHVEQLVESVFKNYDPEGRGTISQE DFERLSGNFPFACHGLHPPPRQGRGSFSREELTGYLLRASAICSKLGLAFLHTFHEVTFRKPTFCDSCSG FLWGVTKQGYRCRECGLCCHKHCRDQVKVECKKRPGAKGDAGPPGAPVPSTPAPHASCGSEENHSYTLSL EPETGCQLRHAWTQTESPHPSWETDTVPCPVMDPPSTASSKLDS TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin