Monoclonal Antibody to a-internexin/NF66 (Clone: 1D2)
Abeomics
Image
Figure-1: Immunofluorescent analysis of rat cerebellum section stained with mouse mAb to α-internexin,(34-1052), dilution 1:5,000 in green, and costained with chicken pAb to calretinin, 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The α-internexin antibody selectively stains neuronal processes, in particular parallel fibers, the axons of granule cells. Calretinin antibody stains interneurons predominantly in the molecular layer of the cerebellum.
Image
Figure-2: Western blot analysis of different tissue lysates using mouse mAb to α-internexin,(34-1052), dilution 1:10,000 in green: [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord and [7] cow spinal cord.(34-1052) antibody reveals the α-internexin protein with apparent molecular weight of 64 to 66 kDa with slight variability among species.
DETAILS
Gene:
INA
App Id:
WB, IF/ICC, IHC
Antigen:
a-internexin/NF66
Gene Id:
9118
Isotype:
Mouse, IgG1
Format Id:
T.C. Sup.
Clone Name:
1D2
Uniprot Id:
Q16352
Host Animal:
Mouse
Clonality Id:
Monoclonal
Reactivity Id:
Human, Cat, Rat, Mouse
Product Content:
Antibody is supplied as an aliquot of 1 mg/ml of affinity purified antibody or concentrated tissue culture supernatant.
Alternative Name:
66 kDa neurofilament protein||Neurofilament 5
Application Note:
WB: 1:10,000. IF/ICC and IHC: 1:5,000.
Storage Condition:
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Immunogen Information:
Purified recombinant rat Alpha -internexin expressed in and purified from E. coli.