APZ-033_25 mcl APZ-033_50 mcl APZ-033_0.2 ml APZ-033-CF_0.2 ml
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Anti-AKAP12 Antibody
Alomone Labs
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DESCRIPTION:
Anti-AKAP12 Antibody (#APZ-033) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Gravin from mouse, rat, and human samples.
DETAILS
- Form: Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
- Host: Rabbit
- Clone: NA
- Label: Unconjugated
- Purity: Affinity purified on immobilized antigen.
- Target: A-kinase anchoring protein 12, A-kinase anchor protein 250 kDa, AKAP250, Gravin, Myasthenia gravis autoantigen gravin, Src-suppressed C kinase substrate, SSeCKS
- Comment: Contact Alomone Labs for technical support and product customization
- Gene Id: 83397
- Isotype: Rabbit IgG
- Homology: Rat - identical; human - 13/14 amino acid residues identical
- Is Toxin: No
- Sequence: (C)GNLTKESPDTNGPK, corresponding to amino acid residues 1639-1652 of mouse AKAP12
- Synonyms: A-kinase anchoring protein 12, A-kinase anchor protein 250 kDa, AKAP250, Gravin, Myasthenia gravis autoantigen gravin, Src-suppressed C kinase substrate, SSeCKS
- Accession: Q9WTQ5
- Clonality: Polyclonal
- Lead Time: 1-2 Business Days
- Reactivity: Human|Rat|Mouse
- Formulation: PBS pH7.4
- Applications: IF|IHC|WB
- Preservative: No Preservative
- Reconstitution: 0.2 ml double distilled water (DDW).
- Blocking Peptide: BLP-PZ033
- Negative Control: BLP-PZ033
- Positive Control: NA
- Immunogen Location: Intracellular, C-terminus
- Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
- Shipping And Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
- Immunogen Source Species: Mouse
- Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
- Applications May Also Work In: IF|IHC|WB
- Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
- Product Page Scientific Background: The A-kinase anchoring protein 12 (AKAP12) belongs to a family of scaffold proteins, which anchor signaling proteins such as PKA in a spatiotemporal manner4. AKAPs generally bind PKA at their N-terminus, while the sequence at their C-terminus determines their spatial location4. To date, more than 50 AKAPs have been identified and perceived to integrate signaling pathways by anchoring multi-protein complexes1.AKAP12 integrates diverse signaling pathways such as PKA, PKC, and β2-adrenergic receptor7,8. Similar to other members of this protein-family, AKAP12 contains a PKA binding domain. In addition, AKAP12 also contains a docking site for PKC2,6 and a myristoyl acid that sequesters it to the plasma membrane7.AKAP12 is widely expressed in a variety of tissues with the highest expression in male and female reproductive organs, skeletal muscle, kidneys, and urinary tract5.One of the most characterized activities of AKAP12 is regulation of the cell cycle3. AKAP12 controls two fundamental cell processes such as the G1/S transition and cytokinesis. In that being said, studies in NIH-3T3 fibroblasts, showed that ectopic expression of AKAP12 reduced cyclin D1 levels and the phosphorylation of its downstream target Rb, and consequently attenuated cell-cycle progression3. Furthermore, in HeLa cells, knockdown of AKAP12 resulted in profound changes in cell morphology, which indicates the inability of cells to divide3.Given that AKAP12 plays a pivotal role in cell cycle regulation, it is not surprising that in some cases it is involved in cancer malignancies. It is now clear that AKAP12 is a key driver of gastrointestinal cancers such as colorectal cancer and hepatocellular carcinomas8.
- Standard Quality Control Of Each Lot: Western blot analysis
- Application Dilutions Western Blot Wb: 1:200
- Antibody Concentration After Reconstitution: 1 mg/ml
- Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: 1:400