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sc-133222 AF594

Scientist.com Supplier

PKLR (E-2) Alexa Fluor® 594

Santa Cruz Biotechnology

DESCRIPTION

mouse monoclonal IgG2a; PKLR Antibody (E-2) is an IgG2a κ mouse monoclonal PKLR antibody (also designated PKLR antibody) that detects the PKLR protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. PKLR Antibody (E-2) is available as both the non-conjugated anti-PKLR antibody form, as well as multiple conjugated forms of anti-PKLR antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. In mammals, four different isoenzymes exist for pyruvate kinase. Based on their tissue distribution, the isoenzymes are designated L-type (for predominant expression in the liver), R-type (for predominant expression in red blood cells), M1-type (for predominant expression in muscle, brain and heart) and M2-type (for predominant expression in fetal tissues). Pyruvate kinases are responsible for catalyzing the final step in glycolysis: the conversion of phosphoenolpyruvate to pyruvate with the coinciding generation of ATP. The PKLR (pyruvate kinase, liver and RBC) gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The R-type isoform, also known as RPK (R-type pyruvate kinase), exists as a tetramer and, when functioning improperly, can result in chronic/hereditary nonspherocytic hemolytic anemia (CNSHA/HNSHA) or pyruvate kinase hyperactivity (also called high red cell ATP syndrome). The L-type isoform, alternatively known as PKL (pyruvate kinase L-type), also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young (MODY).

DETAILS

  • Host: mouse
  • Type: Primary
  • Uniprot: P30613
  • Clonality: Monoclonal
  • Conjugate: AF594
  • Applications: WB(RGB), IF, IHC(P), FCM
  • Human Gene Id: 5313
  • Mouse Gene Id: 18770
  • Protein Target: PKLR
  • Human Gene Name: PKLR
  • Mouse Gene Name: Pklr
  • Species Reactivity: mouse, rat, human