DESCRIPTION

• Aa: full length
• Mw: 64 kDa (CDK4), 59 kDa (Cyclin D3)
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_000075/NM_001760
• Species: Human
• Uniprot: CDK4: P11802; CyclinD3: P30281
• Shiptemp: -80°C (dry ice)
• Synonyms: Cyclin-Dependent Kinase 4, CDK4: CMM3; PSK-J3; MGC14458 Cyclin D3: CCND3
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase-Serine/Threonine/Protein
• Background: CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex (1). CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2 (2).
• References: 1. Harbour, J W. et al: Cdk phosphorylation triggers sequential intramolecular interactions that progressively block Rb functions as cells move through G1. Cell 98: 859-869, 1999.2. Matsuura, I. et al: Cyclin-dependent kinases regulate the antiproliferative function of Smads. Nature 430: 226-231, 2004.
• Description: Complex of recombinant human CDK4 and CyclinD3, full length, with N-terminal GST-tags (GenBank Accession No. NM_000075, and NM_001760 respectively), co-expressed by baculovirus in Sf9 cells
• Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 40094, 100052, 79674
• Specific Activity: 14 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Kinase-Serine/Threonine