DESCRIPTION

• Category: ZYMO KITS
• Keywords: R2057, direct-zol, directzol, trizol kit, trizol
• Paragraph 1: The Direct-zol™-96 RNA provides a streamlined method for the purification of up to 10 µg (per well) of high-quality RNA directly from samples in TRI Reagent®. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product. The extraction method inactivates viruses and other infectious agents. The procedure is easy: simply apply a sample in TRI Reagent® to the Zymo-Spin™ I­96 Plate, then spin, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.
• Paragraph 2: Sample sources: Cells from culture, solid tissue, plasma, serum, whole blood, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA) or samples stored and preserved in TRI Reagent®, TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol reagents.
• Bullet Point 1: Quick, 96-well purification of high-quality total RNA directly from TRIzol®, TRI Reagent®, or similar
• Bullet Point 2: Bypasses phase separation and precipitation procedures
• Bullet Point 3: RNA is eluted into as little as 10 µl DNase/RNase-free water
• Bullet Point 4: The RNA binding capacity of the Zymo-Spin™ I-96 Plate is 10 µg/well
• Bullet Point 5: A260/A280 > 1.8, A260/A230 > 1.8. Complete removal of DNA can be performed with DNase I digestion
• Bullet Point 6: TRI Reagent® provided 4x 100 ml
• Manufacturer Sku: R2057