Anti-c-Src (N-terminal region) Antibody

DETAILS

Form: Protein A Purified
Gene: SRC
Host: Mouse
Rrid: AB_2196152
Type: Primary Antibody
Clone: M259
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: c-Src (N-terminal region)
Isotype: IgG1
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: Src
Clonality: Mouse Monoclonal
Immunogen: Clone (M259) was generated from a recombinant protein containing amino acids in the N-terminal region of human c-Src. This sequence has high homology to similar regions in rat and mouse c-Src.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody detects a 60 kDa* protein corresponding to c-Src on SDS-PAGE immunoblots of mouse SYF cells transformed with c-Src and human A431 cells.
Applications: WB, IP
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 60
Dilution Range Ip: 1:100
Dilution Range Wb: 1:1000
Immunogen Species: Human
Species Reactivity: Human, Mouse, and Rat
Target Description: c-Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. The Src family is composed of nine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an N-terminal cell membrane anchor followed by SH3 and SH2 domains. The activity of c-Src is regulated by tyrosine phosphorylation at multiple sites. Tyrosine 418 is autophosphorylated following c-Src activation. Tyrosine 215 in the SH2 domain of c-Src is phosphorylated following growth factor receptor activation. Both Tyr-215 and Tyr-418 phosphorylation increases tyrosine kinase activity, while phosphorylation of Tyr-530 downregulates c-Src kinase activity. Thus, tyrosine phosphorylation of c-Src is critical for regulating its kinase activity.
Uniprot Number Immunogen Species: P12931