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SMC-416D-RPE

Scientist.com Supplier

Dicer Antibody, Clone N167/7: RPE

Stressmarq Biosciences

DESCRIPTION

Mouse Anti-Mouse Dicer Monoclonal IgG1

DETAILS

  • Target: Dicer
  • Gene Id: 192119
  • Isotype: IgG1
  • Conjugate: RPE
  • Immunogen: Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer
  • References: 1. Hutvágner G. and Zamore P.D. (2002) Science 297: 2056-60. 2. Lee Y., et al. (2003) Nature 425: 415-9. 3. Diederichs S. and Haber, D.A. (2007) Cell 131: 1097-108.
  • Swiss-prot: Q8R418
  • Specificity: Detects ~215kDa.
  • Applications: WB | ICC/IF
  • Clone Number: N167/7 (Formerly sold as S167-7)
  • Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
  • Host Species: Mouse
  • Product Type: Monoclonal
  • Purification: Protein G Purified
  • Concentration: 1 mg/ml
  • Research Areas: Cell Signaling | Epigenetics and Nuclear Signaling
  • Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
  • Accession Number: NP_683750.2
  • Alternative Names: Dicer1 Antibody, DCR Antibody, DCR1 Antibody, DCR-1 Antibody, Double-strand-specific ribonuclease Antibody, Helicase with RNase motif Antibody, HERNA Antibody, Helicase MOI Antibody, KIAA0928 Antibody, Endoribonuclease Dicer Antibody
  • Antibody Dilution: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Cite This Product: StressMarq Biosciences Cat# SMC-416D-RPE, RRID: AB_2701251
  • Immunogen Species: Mouse
  • Storage Temperature: Conjugated antibodies should be stored according to the product label
  • Shipping Temperature: Blue Ice or 4ºC
  • Cellular Localization: Cytoplasm
  • Scientific Background: Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3)
  • Certificate of Analysis: 1 µg/ml of SMC-416 was sufficient for detection of Dicer in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Species Reactivity Full Name: Human | Mouse | Rat