SMC-416D-RPE
Scientist.com Supplier
Dicer Antibody, Clone N167/7: RPE
Stressmarq Biosciences
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DESCRIPTION
Mouse Anti-Mouse Dicer Monoclonal IgG1
DETAILS
- Target: Dicer
- Gene Id: 192119
- Isotype: IgG1
- Conjugate: RPE
- Immunogen: Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer
- References: 1. Hutvágner G. and Zamore P.D. (2002) Science 297: 2056-60. 2. Lee Y., et al. (2003) Nature 425: 415-9. 3. Diederichs S. and Haber, D.A. (2007) Cell 131: 1097-108.
- Swiss-prot: Q8R418
- Specificity: Detects ~215kDa.
- Applications: WB | ICC/IF
- Clone Number: N167/7 (Formerly sold as S167-7)
- Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
- Host Species: Mouse
- Product Type: Monoclonal
- Purification: Protein G Purified
- Concentration: 1 mg/ml
- Research Areas: Cell Signaling | Epigenetics and Nuclear Signaling
- Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
- Accession Number: NP_683750.2
- Alternative Names: Dicer1 Antibody, DCR Antibody, DCR1 Antibody, DCR-1 Antibody, Double-strand-specific ribonuclease Antibody, Helicase with RNase motif Antibody, HERNA Antibody, Helicase MOI Antibody, KIAA0928 Antibody, Endoribonuclease Dicer Antibody
- Antibody Dilution: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
- Cite This Product: StressMarq Biosciences Cat# SMC-416D-RPE, RRID: AB_2701251
- Immunogen Species: Mouse
- Storage Temperature: Conjugated antibodies should be stored according to the product label
- Shipping Temperature: Blue Ice or 4ºC
- Cellular Localization: Cytoplasm
- Scientific Background: Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3)
- Certificate of Analysis: 1 µg/ml of SMC-416 was sufficient for detection of Dicer in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Species Reactivity Full Name: Human | Mouse | Rat