DESCRIPTION

• Aa: 2-939 (end)
• Mw: 130 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_001143848
• Species: Mouse
• Uniprot: O00408
• Shiptemp: -80°C (dry ice)
• Warnings: Avoid freeze/thaw cycles.
• Category: Phosphodiesterase/Enzymes
• References: 1. Sadhu, K., et al., JHC 47(7), 889-894 (1999). 2. Iffland, A., et al., Biochemistry 44 (23), 8312-8325 (2005).
• Description: Murine PDE2A, variant 1 (GenBank Accession No. NM_001143848), a.a 2-939 (end), with N-terminal GST tag, MW=132 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20 and 20% glycerol.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3ʹ, 5ʹ-cAMP to 5ʹ-AMP per min at 37°C.
• Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of PDE2A at 37°C for 20 min. Quantified by 5ʹ nucleotidase cleaving the 5ʹ-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
• Related Products: 60321, 60373, 60062, 60018
• Specific Activity: ≥20,000 pmole/min/µg
• Storage Stability: At least 6 months at -80°C. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
• Scientific Category: Phosphodiesterase