79054
GITR:GITRL TR-FRET Assay Kit
BPS Bioscience
DESCRIPTION
The GITR:GITRL TR-FRET Assay is designed to measure the inhibition of GITR binding to GITRL in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated GITR, GITRL, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.
DETAILS
- Uniprot: Q7TS55
- Shiptemp: -80°C (dry ice)
- Synonyms: Glucocorticoid-induced TNFR-Related Protein, Tumor Necrosis Factor Receptor Superfamily member 18, TNFRSF18,
- Warnings: Avoid freeze/thaw cycles.
- Category: Immunotherapy/Assay Kit
- References: 1. Lechner, M.G., et al. Immunotherapy. 2011 Nov; 3(11): 1317-1340. 2. Ko, K., et al., J. Exp. Med. 2005; 202(7): 885-891.
- Description: The GITR:GITRL TR-FRET Assay is designed to measure the inhibition of GITR binding to GITRL in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated GITR, GITRL, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.
- Methodology: TR-FRET
- Unspsc Code: 41121811
- Unspsc Name: Laboratory microchemistry kits
- Applications: Great for screening small molecular inhibitors for drug discovery and HTS applications.
- Product Type: Assay Kit
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 72061, 71295-1, 71295-2, 60546, 60547, 79092
- Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
- Scientific Category: Immunotherapy
- Instructions for Use: See assay kit datasheet for detailed protocol.