DESCRIPTION

• Aa: 1-261(end)
• Mw: 29.7 kDa
• Tags: C-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: NM_001128829
• Species: Human
• Uniprot: P00915
• Shiptemp: -80°C (dry ice)
• Synonyms: carbonic anhydrase I, CA1
• Warnings: Avoid freeze/thaw cycles.
• Category: Metabolic Enzymes-Anhydrase
• References: 1. Lowe, N., et al., Gene 1990 Sep 14;93(2):277-83.2. Jover, T., et al., J Neurosci. 2002 Mar 15;22(6):2115-24.
• Description: Human carbonic anhydrase I, also known as CA1, (GenBank Accession No. NM_001128829), full length (a.a. 1-261(end)) with C-terminal His-tag, MW = 29.7 kDa, expressed in an E. coli cell expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 185 mM imidazole and 20% glycerol
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Product Type: Protein
• Host Cell Line: E. coli
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: 15 mM Tris-HCl, pH 7.4, 100 mM NaCl, 3 mM p-Nitrophenyl acetate (PNPA) and 0-2 µg CA1. Incubate for 30 minutes at room temperature. Absorbance is measured at 405 nm.
• Specific Activity: 2,000 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Metabolic Enzymes-Anhydrase
• Instructions for Use: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.