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71093-1 71093-2

Carbonic Anhydrase 1 (CA1), His-tag Recombinant

BPS Bioscience

DESCRIPTION

Human carbonic anhydrase I, also known as CA1, (GenBank Accession No. NM_001128829), full length (a.a. 1-261(end)) with C-terminal His-tag, MW = 29.7 kDa, expressed in an E. coli cell expression system.

DETAILS

  • Aa: 1-261(end)
  • Mw: 29.7 kDa
  • Tags: C-terminal His-tag
  • Format: Aqueous buffer solution
  • Genbank: NM_001128829
  • Species: Human
  • Uniprot: P00915
  • Shiptemp: -80°C (dry ice)
  • Synonyms: carbonic anhydrase I, CA1
  • Warnings: Avoid freeze/thaw cycles.
  • Category: Metabolic Enzymes-Anhydrase
  • References: 1. Lowe, N., et al., Gene 1990 Sep 14;93(2):277-83.2. Jover, T., et al., J Neurosci. 2002 Mar 15;22(6):2115-24.
  • Description: Human carbonic anhydrase I, also known as CA1, (GenBank Accession No. NM_001128829), full length (a.a. 1-261(end)) with C-terminal His-tag, MW = 29.7 kDa, expressed in an E. coli cell expression system.
  • Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 185 mM imidazole and 20% glycerol
  • Unspsc Code: 12352202
  • Unspsc Name: Proteins
  • Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
  • Product Type: Protein
  • Host Cell Line: E. coli
  • Biosafety Level: Not applicable (BSL-1)
  • Assay Conditions: 15 mM Tris-HCl, pH 7.4, 100 mM NaCl, 3 mM p-Nitrophenyl acetate (PNPA) and 0-2 µg CA1. Incubate for 30 minutes at room temperature. Absorbance is measured at 405 nm.
  • Specific Activity: 2,000 pmol/min/µg
  • Storage Stability: At least 6 months at -80°C.
  • Scientific Category: Metabolic Enzymes-Anhydrase
  • Instructions for Use: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.