DESCRIPTION

• Shiptemp: -80°C (dry ice)
• Synonyms: TDO2, tryptophan 2,3 dioxygenase
• Warnings: Avoid freeze/thaw cycles.
• Category: Immunotherapy/Assay Kit
• Background: L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Tryptophan 2,3 dioxygenase (TDO) is upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
• References: Li, J.S., et al. Arch. Insect Biochem. Physiol. 2007 Feb; 64(2): 74-87.
• Description: The TDO Inhibitor Screening Assay Kit is designed to measure TDO enzyme inhibition. The TDO Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation, activity is measured by measuring the absorption of reaction product at _x0001_ = 320-325 nm.
• Methodology: Colorimetric
• Supplied As: The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 400 reactions.
• Unspsc Code: 41121811
• Unspsc Name: Laboratory microchemistry kits
• Applications: Useful for the study of TDO enzymology, screening inhibitors and selectivity profiling.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 71195, 72023, 72033, 60534, 72039
• Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
• Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Scientific Category: Immunotherapy
• Instructions for Use: See assay kit datasheet for detailed protocol.