CT048-H27H-B
Human IL23 Protein (His & AVI Tag), Biotinylated
Sino Biological
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DESCRIPTION
A DNA sequence encoding the human IL23 complex composed of human IL12B (NP_002178.2) (Met1-Ser328) and human IL23A (NP_057668.1) (Arg20-Pro189) linked by a polypeptide linker was fused with a C-terminal AVI tag followed by a polyhistidine tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
DETAILS
- Tag: C-AVI & His
- Purity: > 95 % as determined by SDS-PAGE.
- Source: HEK293 Cells
- Species: Human
- Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: Immobilized Human IL23 Receptor Protein (ECD, hFc Tag) (Cat:13840-H02H) at 2 μg/mL (100 μL/well) can bind Human IL-23 Protein (His & AVI Tag), Biotinylated (Cat:CT048-H27H-B), the EC50 is 180-540 ng/mL.
- Molecule: IL12B & IL23A
- Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
- Lead Time: 1-3 days
- Formulation: Supplied as sterile 50 mM Tris, 100 mM Nacl, 10%glycerol, 0.02% Tween 80, 10% Trehalose, pH 8.5.
- Molecular Mass: The recombinant human IL23 complex (IL23A&IL12B) consists of 517 amino acids and predicts a molecular mass of 57.6 kDa.
- Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
- Accession Number: NP_002178.2 & NP_057668.1
- Predicted N Terminal: Ile 23