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CT048-H27H-B

Scientist.com Supplier

Human IL23 Protein (His & AVI Tag), Biotinylated

Sino Biological

DESCRIPTION

A DNA sequence encoding the human IL23 complex composed of human IL12B (NP_002178.2) (Met1-Ser328) and human IL23A (NP_057668.1) (Arg20-Pro189) linked by a polypeptide linker was fused with a c-terminal AVI tagged polyhistidine tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

DETAILS

  • Tag: C-AVI & His
  • Purity: > 95 % as determined by SDS-PAGE.
  • Source: HEK293 Cells
  • Species: Human
  • Storage: Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Activity: 1.Immobilized Recombinant Human IL23 Protein (His & AVI Tag), Biotinylated (Cat: CT048-H27H-B ) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human IL23R / IL23 Receptor Protein (ECD, Fc Tag) (Cat: 13840-H02H) , the EC50 is 8-30 ng/mL. 2.Immobilized Recombinant Human IL23 Protein (His & AVI Tag), Biotinylated (Cat: CT048-H27H-B)at 2 μg/mL (100 μL/well) can bind Recombinant Human IL12RB1 Protein (ECD, Fc Tag) (Cat: 11674-H02H), the EC50 is 1-3 ng/mL (Routinely tested). 3.Loaded Biotinylated Recombinant Human IL-23 Protein, His & AVI Tag (Cat. No. CT048-H27H-B) on SA Biosensor, can bind Recombinant Human IL-23 Receptor Protein, hFc Tag (Cat. No. 13840-H02H) with an affinity constant of 65.7 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
  • Category: Proteins
  • Molecule: IL12B & IL23A
  • Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
  • Lead Time: 1-3 days
  • Formulation: Supplied as sterile 50 mM Tris, 100 mM Nacl, 10%glycerol, 0.02% Tween 80, 10% Trehalose, pH 8.5.
  • Molecular Mass: The recombinant human IL23 complex (IL23A&IL12B) consists of 517 amino acids and predicts a molecular mass of 57.6 kDa.
  • Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Accession Number: NP_002178.2 & NP_057668.1
  • Predicted N Terminal: Ile 23