B1370-189004
Proteinase K
BOC Sciences
DETAILS
- Iupac: (4-nitrophenyl)methyl (4R,5S,6S)-6-[(1R)-1-hydroxyethyl]-4-methyl-7-oxo-3,4-diphenoxy-5-phosphonooxy-1-azabicyclo[3.2.0]hept-2-ene-2-carboxylate
- Smiles: CC(C1C(=O)N2C1(C(C(=C2C(=O)OCC3=CC=C(C=C3)[N+](=O)[O-])OC4=CC=CC=C4)(C)OC5=CC=CC=C5)OP(=O)(O)O)O
- Aliases: EC 3.4.21.64; endopeptidase K; Tritirachium alkaline proteinase; Tritirachium album serine proteinase; Tritirachium album proteinase K
- Cas Number: 39450-01-6
- Molecular Weight: 626.50
- Molecular Formula: C29H27N2O12P
DESCRIPTION
Proteinase K is a subtilisin-related serine protease. It was discovered from the fungus Engyodontium album. It can be used for removal of endogenous nucleases during the preparation of DNA and RNA, and for preparation of tissue sections for in situ hybridization. It is often used in pH range 7.5-9.0 and 37-70°C. Specific activity is ≥ 34 units/mg of protein.