DESCRIPTION

• Mw: 29.3 kDa
• Tag: Myc-DDK
• Accn: NM_001145277
• Type: Human Tagged ORF Clone
• Refseq: NM_001145277.1, NP_001138749.1
• Status: In Stock*
• Symbol: NECAP2
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 55707
• Orf Size: 819 bp
• Refseq Orf: 822 bp
• Uniprot Id: Q9NVZ3
• Availability: In Stock
• Cytogenetics: 1p36.13
• Gene Summary: This gene likely encodes a member of the adaptin-ear-binding coat-associated protein family. Studies of a similar protein in rat suggest a role in clathrin-mediated endocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009]
• Chromatograms: CHROMATOGRAMS Sequencher program is needed, download here.
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC227659 representing NM_001145277 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGAGGAGAGCGGGTACGAGTCGGTGCTCTGTGTCAAGCCTGACGTCCACGTCTACCGCATCCCTCCGC GGGCTACCAACCGTGGCTACAGGGCTGCGGAGTGGCAGCTGGACCAGCCATCATGGAGTGGCCGGCTGAG GATCACTGCAAAGGGACAGATGGCCTACATCAAGCTGGAGGACAGGACGTCAGGGGAGCTCTTTGCTCAG GCCCCGGTGGATCAGTTTCCTGGCACAGCTGTGGAGAGTGTGACGGATTCCAGCAGGTACTTCGTGATCC GCATCGAAGATGGAAATGGGCGACGGGCGTTTATTGGAATTGGCTTCGGGGACCGAGGTGATGCCTTTGA CTTCAATGTTGCATTGCAGGACCATTTCAAGTGGGTGAAACAGCAGTGTGAATTTGCAAAACAAGCCCAG AACCCAGACCAAGGCCCTAAACTGGACCTGGGCTTCAAGGAGGGCCAGACCATCAAGCTCAACATCGCAA ACATGAAGAAGAAGGAAGGAGCAGCTGGGAATCCCCGAGTCCGGCCTGCCAGCACAGGAGGGCTGAGCCT GCTTCCCCCTCCCCCAGGGGGGAAAACCTCCACCCTGATCCCTCCCCCTGGGGAGCAGTTGGCTGTGGGG GGATCCCTCGTCCAGCCAGCAGTTGCTCCCAGTTCAGATCAACTTCCAGCCAGACCCAGCCAGGCACAGG CTGGGTCCAGTTCTGACCTGAGCACGGTTTTTCCTCATGTGACTTCTGGGAAGGCGCTCCCTCATCTGGG CCAAAGGAAGGAGGACGAAGCCCTCCTCAGCTGGCCTGTGTTTGGGGCA ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC227659 representing NM_001145277 Red =Cloning site Green =Tags(s) MEESGYESVLCVKPDVHVYRIPPRATNRGYRAAEWQLDQPSWSGRLRITAKGQMAYIKLEDRTSGELFAQ APVDQFPGTAVESVTDSSRYFVIRIEDGNGRRAFIGIGFGDRGDAFDFNVALQDHFKWVKQQCEFAKQAQ NPDQGPKLDLGFKEGQTIKLNIANMKKKEGAAGNPRVRPASTGGLSLLPPPPGGKTSTLIPPPGEQLAVG GSLVQPAVAPSSDQLPARPSQAQAGSSSDLSTVFPHVTSGKALPHLGQRKEDEALLSWPVFGA TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin