APZ-009-GP_25 mcl APZ-009-GP_50 mcl APZ-009-GP_0.2 ml APZ-009-GP-CF_0.2 ml
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Guinea Pig Anti-PSD-95 Antibody
Alomone Labs
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DESCRIPTION:
Guinea Pig Anti-PSD-95 Antibody (#APZ-009-GP, formerly AGP-132) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot applications. It has been designed to recognize PSD-95 from rat, mouse, and human samples. The antigen used to immunize guinea pigs is the same as Anti-PSD-95 Antibody (#APZ-009) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.
DETAILS
- Form: Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
- Host: Guinea Pig
- Clone: NA
- Label: Unconjugated
- Purity: Affinity purified on immobilized antigen.
- Target: Postsynaptic density protein 95, Discs large homolog 4, DLG4, Synapse-associated protein 90, SAP-90, Tax interaction protein 15, MRD62
- Comment: Contact Alomone Labs for technical support and product customization
- Gene Id: 1742
- Isotype: Guinea pig total IgG
- Homology: Rat, mouse - identical
- Is Toxin: No
- Sequence: (C)KDWGSSSGSQGRED, corresponding to amino acid residues 505-518 of human PSD-95
- Synonyms: Postsynaptic density protein 95, Discs large homolog 4, DLG4, Synapse-associated protein 90, SAP-90, Tax interaction protein 15, MRD62
- Accession: P78352
- Clonality: Polyclonal
- Lead Time: 1-2 Business Days
- Reactivity: Human|Rat|Mouse
- Formulation: PBS pH7.4
- Applications: WB
- Preservative: No Preservative
- Reconstitution: 0.2 ml double distilled water (DDW).
- Blocking Peptide: BLP-PZ009
- Negative Control: BLP-PZ009
- Positive Control: NA
- Immunogen Location: Intracellular, between the SH3 and the GK-like domains.
- Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
- Shipping And Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
- Immunogen Source Species: Human
- Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
- Applications May Also Work In: WB
- Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
- Product Page Scientific Background: The postsynaptic density (PSD) is a membrane-associated protein specialized in postsynaptic signal transduction and processing. There are four PSD family members classified according to their molecular weight, including, PSD-95, PSD-93, synaptic associated proteins 97 kDa and 102 kDa. PSD-95 interacts with both ionotropic and metabotropic glutamate receptors via protein-protein interactions and plays a role in their precise assembly and spatial organization as well as coupling of these receptors to downstream signaling events1.PSD-95 contains a SH3 domain, a GK domain and three PDZ domains. The PDZ domains 1 and 2 of PSD-95 are separated by only a few residues, creating a rigid bond that may restrain interdomain flexibility. These two PDZ domains can have distinct or overlapping target-binding proteins. In contrast, PDZ3 of PSD-95 is located at the carboxyl terminal end of the protein and has a set of interacting proteins that are distinct from PDZ domains 1 and 2. The N-palmitoylation of PSD-95 at Cys-3 and Cys-5 residues is essential for enhanced synaptic responses and the trafficking of PSD-95 to the plasma membrane2. With its numerous domains, PSD-95 is often referred to as a scaffold protein, which localizes and traffics various receptors, cell adhesion molecules, ion channels, kinases and phosphatases to the synaptic membrane. PSD-95 also interacts with a variety of adaptor and cytoskeleton proteins, molecular motors, as well as protein synthesis machinery3.PSD-95 is found at the postsynaptic membrane of excitatory synapses4.PSD-95 null mice have severe learning defects and exhibit both facilitation of long-term potentiation and disruption of long-term depression5.
- Standard Quality Control Of Each Lot: Western blot analysis
- Application Dilutions Western Blot Wb: 1:200
- Antibody Concentration After Reconstitution: 1 mg/ml
- Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: Contact Alomone for information