Bmf Antibody

DETAILS

Buffer: Bmf Antibody is supplied in PBS containing 0.02% sodium azide.
Isotype: IgG
Storage: Bmf antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Category: Antibody
Homology: Predicted species reactivity based on immunogen sequence: Rat: (92%)
Shipping: Blue Ice
Clonality: Polyclonal
Conjugate: Unconjugated
Immunogen: Bmf antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human Bmf.The immunogen is located within the first 50 amino acids of Bmf.
Swissprot: Q96LC9
User Note: Optimal dilutions for each application to be determined by the researcher.
Background: Bmf Antibody: Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program. Bmf is constitutively expressed in many tissues.
Host Species: Rabbit
Ncbi Gene Id: 90427
Purification: Bmf Antibody is affinity chromatography purified via peptide column.
Concentration: 1 mg/mL
Modifications: None
Ncbi Organism: Homo sapiens
Physical State: Liquid
Additional Names: Bmf Antibody: Bcl-2-modifying factor
Application Note: Bmf antibody can be used for detection of Bmf by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
By Research Area: Apoptosis
Protein Accession: NP_277038
Protein Gi Number: 15723378
Ncbi Official Name: Bcl2 modifying factor
Positive Control 1: Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2: Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 3: Cat. No. 1201 - HeLa Cell Lysate
Positive Control 4: Cat. No. 17-001 - HeLa Cell Slide
Species Reactivity: Human,Mouse
Tested Applications: ELISA,WB,ICC
Ncbi Official Symbol: BMF
Predicted Molecular Weight: Predicted: 20 kDa Observed: 22 kDa