DESCRIPTION

• Category: ZYMO KITS
• Keywords: R2050S, R2050, directzol, directsol, direct zol, acid-guanidinium-phenol, direct RNA prep, direct RNA purification, RNA purification, Z5227
• Paragraph 1: The Direct-zol™ RNA MiniPrep provides a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI-Reagent® or similar*. Total RNA (including small and non-coding RNAs) is effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.) using this product. The procedure is easy: simply apply a sample in TRI-Reagent® to spin column and then spin, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. Small RNAs ( >= 17 nucleotides) are efficiently and consistently recovered using this kit. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc. The entire procedure typically takes about 5-10 minutes.
• Paragraph 2: The RNA binding capacity of the supplied Zymo-Spin™ IIC Column is ~50 µg with a minimal elution volume of 25 µl.
• Bullet Point 1: Isolate small and large RNAs directly from TRIzol® or TRI Reagent® without phase separation in 10 minutes!
• Bullet Point 2: Quick, spin column purification of high-quality total RNA (17 nt or greater)
• Bullet Point 3: No phase separation. No precipitation. No phenol carryover. No hassle. No mess!
• Bullet Point 4: High-Quality, DNA-FREE RNA for all downstream applications
• Bullet Point 5: Ideal for cells, tissues, biological liquids, in vitro transcripts, etc
• Bullet Point 6: Enhanced recovery of small and miRNAs that are normally more difficult to isolate
• Bullet Point 7: High quality RNA (A260/A280 <1.8, A260/A230 <1.8) is recovered
• Bullet Point 8: Processing Time: 10 min
• Manufacturer Sku: R2050