SP1371
Anti-c-Src (Tyr-215) [conserved site], Phosphospecific Antibody
PhosphoSolutions
DETAILS
- Form: Antigen Affinity Purified
- Gene: SRC
- Host: Rabbit
- Rrid: AB_2196149
- Type: Primary Antibody
- Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
- Target: c-Src (Tyr-215)
- Isotype: IgG
- Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
- Shipping: Blue Ice
- Synonyms: Src
- Clonality: Rabbit Polyclonal
- Immunogen: c-Src (Tyr-215) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 215 of human c-Src. This sequence has homology to the conserved site in other Src family members, such as hck, fyn, csk, and yes.
- Expiration: After date of receipt, stable for at least 1 year at -20°C.
- Specificity: This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-c-Src (Tyr-215) peptide (without carrier). The antibody detects a 60 kDa* protein on SDS-PAGE immunoblots of mouse SYF cells transformed with c-Src and human A431 cells that have been treated with pervanadate.
- Applications: WB
- Conjugate Exem: Unconjugated
- Physical State: Liquid
- Quality Control: Western blots performed on each lot.
- Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
- Molecular Weight: 60
- Dilution Range Wb: 1:1000
- Immunogen Species: Human
- Species Reactivity: Human, Mouse, and Rat
- Target Description: c-Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. The Src family is composed of nine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an N-terminal cell membrane anchor followed by SH3 and SH2 domains. The activity of c-Src is regulated by tyrosine phosphorylation at multiple sites. Tyrosine 418 is autophosphorylated following c-Src activation. Tyrosine 215 in the SH2 domain of c-Src is phosphorylated following growth factor receptor activation. Both Tyr-215 and Tyr-418 phosphorylation increases tyrosine kinase activity, while phosphorylation of Tyr-530 downregulates c-Src kinase activity. Thus, tyrosine phosphorylation of c-Src is critical for regulating its kinase activity.
- Uniprot Number Immunogen Species: P12931
- Post Translational Modification Type: Phosphorylated
- Post Translational Modification Residue: Tyr-215