SEK10199
Human KLK13 / Kallikrein 13 ELISA Pair Set
Sino Biological
Image
DESCRIPTION
The human KLK13 / Kallikrein 13 ELISA Pair Set is for the quantitative determinationof human KLK13 / Kallikrein 13.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is asolid phase sandwich ELISA (Enzyme-Linked ImmunosorbentAssay). It utilizes a monoclonal antibody specific for KLK13 / Kallikrein 13 coated on a 96-well plate. Standards and samples areadded to the wells, and any KLK13 / Kallikrein 13 presentbinds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-KLK13 / Kallikrein 13 polyclonal antibody is then added, producing anantibody-antigen-antibody "sandwich". The wells are again washed and TMBsubstrate solution is loaded, which produces color in proportion to the amountof KLK13 / Kallikrein 13 presentin the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
DETAILS
- Storage: Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
- Standard: Each vial contains 110 ng of recombinant KLK13. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.
- Lead Time: 1-2 weeks
- Application: ELISA
- Assay Range: 39.06-2500
- Specificity: Human KLK13 / Kallikrein 13
- Capture Antibody: 0.25 mg/mL of mouse anti-KLK13 monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating. (Catalog: # 10199-MM06)
- Detection Antibody: 0.5 mg/mL rabbit anti-KLK13 polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.