40589-V49H7-B
SARS-CoV-2 (BA.2.12.1) Spike S1+S2 trimer Protein (ECD, His & AVI Tag), Biotinylated
Sino Biological
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DESCRIPTION
A DNA sequence encoding the SARS-CoV-2 (BA.2.12.1) Spike S1+S2 trimer (YP_009724390.1, with mutations T19I, L24S, del25-27, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, S704L, N764K, D796Y, F817P, A892P, A899P, A942P, Q954H, N969K, K986P, V987P and furin cleavage site mutants) was expressed with bacteriophage T4 fibritin and a C-terminal polyhistidine tag followed by an AVI tag. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.2.12.1).The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
DETAILS
- Tag: C-His-AVI
- Purity: > 90 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
- Source: HEK293 Cells
- Species: SARS-CoV-2
- Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: Immobilized human ACE2 protein (Fc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 (BA.2.12.1) Spike S1+S2 trimer Protein(ECD,His&AVI Tag) (Cat: 40589-V49H7-B), the EC50 is 3-10 ng/mL.
- Molecule: Spike
- Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
- Lead Time: 1-3 days
- Formulation: Lyophilized from a sodium citrate buffer system at pH 6.0.
- Molecular Mass: The recombinant SARS-CoV-2 (BA.2.12.1) Spike S1+S2 trimer consists of 1245 amino acids and predicts a molecular mass of 138.61 kDa.
- Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Accession Number: YP_009724390.1
- Predicted N Terminal: Val 16