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MR218457L4

Scientist.com Supplier

Vps8 (NM_001081366) Mouse Tagged Lenti ORF Clone

Origene Technologies, Inc.

DESCRIPTION

Lenti ORF clone of Vps8 (mGFP-tagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (Vps8)

DETAILS

  • Tag: mGFP
  • Accn: NM_001081366
  • Type: Mouse Tagged ORF Clone
  • Refseq: NM_001081366.1, NP_001074835.1
  • Status: 6 Weeks*
  • Symbol: Vps8
  • Vector: pLenti-C-mGFP-P2A-Puro
  • Category: cDNA Clones
  • Locus Id: 209018
  • Orf Size: 4284 bp
  • Synonyms: AI315068; AU040738; mKIAA0804
  • Refseq Orf: 4290 bp
  • Refseq Size: 5032 bp
  • Availability: 6 Weeks
  • Cytogenetics: 16 B1
  • Gene Summary: Plays a role in vesicle-mediated protein trafficking of the endocytic membrane transport pathway. Believed to act as a component of the putative CORVET endosomal tethering complexes which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Functions predominantly in APPL1-containing endosomes (By similarity).[UniProtKB/Swiss-Prot Function]
  • Sequence Data: ORF Nucleotide Sequence ( show hide ) The ORF insert of this clone is exactly the same as(MR218457).
  • Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
  • Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
  • Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
  • E Coli Selection: Chloramphenicol (34 ug/mL)
  • Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
  • Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
  • Mammalian Cell Selection: Puromycin