DESCRIPTION

• Target: p53
• Gene Id: 7157
• Conjugate: ATTO 390
• Immunogen: Synthetic peptide from the C-terminal of human tumor protein p53.
• References: 1. Georescu C.V., Saftoiu A., Georgescu C.C., Ciurea R. and Ciurea T. (2007) J Gastrointestin Liver Dis. 16(2): 133-9. 2. Strachan T., et al. (1999) Human Molecular Genetics 2. Ch. 18: Cancer Genetics. 3. Hansen J.E., et al. (2007) Cancer Research 67: 1769-1774. 4. Di Cesare E., et al. (2001) Ann Clin Lab Sci 31: 253-8. 5. Akere A., Otegbayo J.A. (2007) Singapore Med J. 48(1): 41-4.
• Swiss-prot: P04637
• Specificity: Predicted molecular weight at ~43.7kDa. Observed bands at ~53kDa.
• Applications: WB | ICC/IF
• Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
• Host Species: Rabbit
• Product Type: Polyclonal
• Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml
• Research Areas: Cancer | Cell Cycle | p53 Pathway | Cancer | Tumor suppressors | Apoptosis | Cell Signaling | Epigenetics and Nuclear Signaling | DNA/RNA | DNA Damage and Repair
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Accession Number: NP_000537.3
• Alternative Names: TP53 Antibody, Tumor Protein 53 Antibody, BCC7 Antibody, LFS1 Antibody, TRP53 Antibody, p53 tumor suppressor Antibody
• Antibody Dilution: WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Cite This Product: StressMarq Biosciences Cat# SPC-682D-A390, RRID: AB_2705646
• Immunogen Species: Human
• Storage Temperature: Conjugated antibodies should be stored according to the product label
• Shipping Temperature: Blue Ice or 4ºC
• Cellular Localization: Cytoplasm | Nucleus | PML body | Endoplasmic Reticulum | Mitochondrion matrix
• Scientific Background: The p53 protein (tumor protein 53 or TP53) is a DNA-binding cell cycle-regulating transcription factor that governs cell division and the fine balance between cell death and cell survival (1). P53 plays a critical role in tumor suppression and hence it is often described as "the guardian of the genome", "the guardian angel gene", or the "master watchman." This also refers to its role in conserving stability by preventing genome mutation (2). Defects in p53 are linked to >50% of human cancers, and restoring p53 function to these cancer cells can induce growth arrest and apoptosis (3). When p53 has been damaged, it can also lead to autoimmune disorders (4, 5).
• Certificate of Analysis: A 1:1000 dilution of SPC-682 was sufficient for detection of p53 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Species Reactivity Full Name: Human