DESCRIPTION

• Mw: 94 kDa
• Tag: Myc-DDK
• Accn: NM_001146015
• Type: Human Tagged ORF Clone
• Refseq: NM_001146015.2
• Status: 4 Weeks*
• Symbol: HURP
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 9787
• Orf Size: 2526 bp
• Synonyms: DLG7; HURP
• Refseq Orf: 2529 bp
• Uniprot Id: Q15398
• Availability: 4 Weeks
• Cytogenetics: 14q22.3
• Gene Summary: Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.[UniProtKB/Swiss-Prot Function]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC228594 representing NM_001146015 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGTCTTCATCACATTTTGCCAGTCGACACAGGAAGGATATAAGTACTGAAATGATTAGAACTAAAATTG CTCATAGGAAATCACTGTCTCAGAAAGAAAATAGACATAAGGAATACGAACGAAATAGACACTTTGGTTT GAAAGATGTAAACATTCCAACCTTGGAAGGTAGAATTCTTGTTGAATTAGATGAGACATCTCAAGGGCTT GTTCCAGAAAAGACCAATGTTAAGCCAAGGGCAATGAAAACTATTCTAGGTGATCAACGAAAACAGATGC TCCAAAAATACAAAGAAGAAAAGCAACTTCAAAAATTGAAAGAGCAGAGAGAGAAAGCTAAACGAGGAAT ATTTAAAGTGGGTCGTTATAGACCTGATATGCCTTGTTTTCTTTTATCAAACCAGAATGCTGTGAAAGCT GAGCCAAAAAAGGCTATTCCATCTTCTGTACGGATTACAAGGTCAAAGGCCAAAGACCAAATGGAGCAGA CTAAGATTGATAACGAGAGTGATGTTCGAGCAATCCGACCTGGTCCAAGACAAACTTCTGAAAAGAAAGT GTCAGACAAAGAGAAAAAAGTTGTGCAGCCTGTAATGCCCACGTCGTTGAGAATGACTCGATCAGCTACT CAAGCAGCAAAGCAGGTTCCCAGAACAGTCTCATCTACCACAGCAAGAAAGCCAGTCACAAGAGCTGCTA ATGAAAACGAACCAGAAGGAAAGGTGCCAAGTAAAGGAAGACCTGCCAAAAATGTAGAAACAAAACCCGA CAAGGGTATTTCTTGTAAAGTCGATAGTGAAGAAAATACTTTGAATTCACAAACTAATGCAACAAGTGGA ATGAATCCAGATGGAGTCTTATCAAAAATGGAAAACTTACCTGAGATAAATACTGCAAAAATAAAAGGGA AGAATTCCTTTGCACCTAAGGATTTTATGTTTCAGCCACTGGATGGTCTGAAGACCTATCAAGTAACACC TATGACTCCCAGAAGTGCCAATGCTTTTTTGACACCCAGTTACACCTGGACTCCTTTAAAAACAGAAGTT GATGAGTCTCAAGCAACAAAAGAAATTTTGGCACAAAAATGTAAAACTTACTCTACCAAGACAATACAGC AAGATTCAAATAAATTGCCATGTCCTTTGGGTCCTCTAACTGTTTGGCATGAAGAACATGTTTTAAATAA AAATGAAGCTACTACTAAAAATTTAAATGGCCTTCCAATAAAAGAAGTCCCATCACTTGAAAGAAATGAA GGTCGAATTGCTCAGCCCCACCATGGTGTGCCATATTTCAGAAATATCCTCCAGTCAGAAACTGAGAAAT TAACTTCACATTGCTTCGAGTGGGACAGGAAACTTGAATTGGACATTCCAGATGATGCTAAAGATCTTAT TCGCACAGCAGTTGGTCAAACAAGACTCCTTATGAAGGAAAGGTTTAAACAGTTTGAAGGACTGGTTGAT GATTGTGAATATAAACGAGGTATAAAGGAGACTACCTGTACAGATCTGGATGGATTTTGGGATATGGTTA GTTTTCAGATAGAAGATGTAATCCACAAATTCAACAATCTGATCAAACTTGAGGAATCTGGGTGGCAAGT CAATAATAATATGAATCATAATATGAACAAAAATGTCTTTAGGAAAAAAGTTGTCTCAGGTATAGCAAGT AAACCAAAACAGGATGATGCTGGAAGAATTGCAGCGAGAAATCGCCTAGCTGCCATAAAAAATGCAATGA GAGAGAGAATTAGGCAGGAAGAATGTGCTGAAACAGCAGTTTCTGTGATACCAAAGGAAGTTGATAAAAT AGTGTTCGATGCTGGATTTTTCAGAGTTGAAAGTCCTGTTAAATTATTCTCAGGACTTTCTGTCTCTTCT GAAGGCCCTTCTCAAAGACTTGGAACACCTAAGTCTGTCAACAAAGCTGTATCTCAGAGTAGAAATGAGA TGGGCATTCCACAACAAACTACATCACCAGAAAATGCCGGTCCTCAGAATACGAAAAGTGAACATGTGAA GAAGACTTTGTTTTTGAGTATTCCTGAAAGCAGGAGCAGCATAGAAGATGCTCAGTGTCCTGGATTACCA GATTTAATTGAAGAAAATCATGTTGTAAATAAGACAGACTTGAAGGTGGATTGTTTATCCAGTGAGAGAA TGAGTTTGCCTCTTCTTGCTGGTGGAGTAGCAGATGATATTAATACTAACAAAAAAGAAGGAATTTCAGA TGTTGTGGAAGGAATGGAACTGAATTCTTCAATTACATCACAGGATGTTTTGATGAGTAGCCCTGAAAAA AATACAGCTTCACAAAATAGCATCTTAGAAGAAGGGGAAACTAAAATTTCTCAGTCAGAACTATTTGATA ATAAAAGTCTCACTACTGAATGCCACCTTCTTGATTCAGTGGGATCTTGCTATGTTGCTCGGGCTGGTCT TGAAGTCCTGGGTTCAAGTGATCCTACCACCTCAGCCTCCCGAGTAGCTGGGACTACAGCCAGGTCTAAA CTGCAG ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC228594 representing NM_001146015 Red =Cloning site Green =Tags(s) MSSSHFASRHRKDISTEMIRTKIAHRKSLSQKENRHKEYERNRHFGLKDVNIPTLEGRILVELDETSQGL VPEKTNVKPRAMKTILGDQRKQMLQKYKEEKQLQKLKEQREKAKRGIFKVGRYRPDMPCFLLSNQNAVKA EPKKAIPSSVRITRSKAKDQMEQTKIDNESDVRAIRPGPRQTSEKKVSDKEKKVVQPVMPTSLRMTRSAT QAAKQVPRTVSSTTARKPVTRAANENEPEGKVPSKGRPAKNVETKPDKGISCKVDSEENTLNSQTNATSG MNPDGVLSKMENLPEINTAKIKGKNSFAPKDFMFQPLDGLKTYQVTPMTPRSANAFLTPSYTWTPLKTEV DESQATKEILAQKCKTYSTKTIQQDSNKLPCPLGPLTVWHEEHVLNKNEATTKNLNGLPIKEVPSLERNE GRIAQPHHGVPYFRNILQSETEKLTSHCFEWDRKLELDIPDDAKDLIRTAVGQTRLLMKERFKQFEGLVD DCEYKRGIKETTCTDLDGFWDMVSFQIEDVIHKFNNLIKLEESGWQVNNNMNHNMNKNVFRKKVVSGIAS KPKQDDAGRIAARNRLAAIKNAMRERIRQEECAETAVSVIPKEVDKIVFDAGFFRVESPVKLFSGLSVSS EGPSQRLGTPKSVNKAVSQSRNEMGIPQQTTSPENAGPQNTKSEHVKKTLFLSIPESRSSIEDAQCPGLP DLIEENHVVNKTDLKVDCLSSERMSLPLLAGGVADDINTNKKEGISDVVEGMELNSSITSQDVLMSSPEK NTASQNSILEEGETKISQSELFDNKSLTTECHLLDSVGSCYVARAGLEVLGSSDPTTSASRVAGTTARSK LQ TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Protein Families: Druggable Genome
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin