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AVR-003_25 mcl AVR-003_50 mcl AVR-003_0.2 ml AVR-003-CF_0.2 ml

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Anti-PACAP Receptor 1 (PAC1) Antibody

Alomone Labs

DESCRIPTION

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat PACAP type I receptor. Anti-PACAP Receptor 1 (PAC1) Antibody (#AVR-003) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PAC1 receptor from rat, mouse and human samples.

DETAILS

  • Form: Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
  • Host: Rabbit
  • Clone: NA
  • Label: Unconjugated
  • Purity: Affinity purified on immobilized antigen.
  • Target: PACAP type I receptor, Pituitary adenylate cyclase-activating polypeptide type I receptor, PACAP-R1, ADCYAP1R1
  • Comment: Contact Alomone Labs for technical support and product customization
  • Gene Id: 24167
  • Isotype: Rabbit IgG
  • Homology: Mouse - identical; human - 12/14 amino acid residues identical
  • Is Toxin: No
  • Sequence: (C)KVNRYFTMDFKHRH, corresponding to amino acid residues 474-487 of rat PAC1 receptor
  • Synonyms: PACAP type I receptor, Pituitary adenylate cyclase-activating polypeptide type I receptor, PACAP-R1, ADCYAP1R1
  • Accession: P32215
  • Clonality: Polyclonal
  • Lead Time: 1-2 Business Days
  • Reactivity: Rat|Mouse
  • Formulation: PBS pH7.4
  • Applications: IF|IHC|WB
  • Ko-validated: yes
  • Preservative: No Preservative
  • Reconstitution: 0.2 ml double distilled water (DDW).
  • Blocking Peptide: BLP-VR003
  • Negative Control: BLP-VR003
  • Positive Control: NA
  • Cited Application: IHC
  • Immunogen Location: Intracellular, C-terminus
  • Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
  • Shipping and Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
  • Immunogen Source Species: Rat
  • Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
  • Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
  • Applications May Also Work In: IF|IHC|WB
  • Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
  • Product Page - Scientific Background: Pituitary adenylate cyclase-activating polypeptide (PACAP, encoded by ADCYAP1) belongs a superfamily of structurally related peptide hormones, which include vasoactive intestinal peptide (VIP), glucagon, glucagon-like peptides, secretin, gastric inhibitory peptide and growth hormone-releasing hormone.PACAP acts through PAC1, VPAC1 and VPAC2, initiating multiple signaling pathways1. PAC1 is a class B member of the G-protein coupled receptors (GPCRs) superfamily. Class B receptors are differentiated from Class A by a relatively large N-terminal ectodomain that is crucial to ligand recognition2.PAC1R is mainly expressed on smooth muscle cells, neurons, endothelial cells and peritoneal macrophages3.PAC1R is associated with posttraumatic stress disorder (PTSD) symptoms and psychophysiological markers of fear and anxiety4.PAC1 receptor null mice demonstrate reduced anxiety-like behavior5. In addition, PAC1 overexpression can result in hydrocephalus- and schizophrenia-related neuropathology1.
  • Standard Quality Control of Each Lot: Western blot analysis
  • Application Dilutions Western Blot Wb: 1:500
  • Antibody Concentration After Reconstitution: 1 mg/ml
  • Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: 1:400