ALM-051-B_50 mcg
Scientist.com Supplier
Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-Biotin Antibody
Alomone Labs
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DESCRIPTION:
Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody (#ALM-051) is a highly specific monoclonal antibody directed against an epitope of the human channel. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize KCNN4 from human, rat, and mouse samples. Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-Biotin Antibody (#ALM-051-B) is directly labeled with biotin. Strepavidin tagged with HRP or with a fluorescent probe can then be used to detect the protein. The biotin/strepavidin system is ideal for minimizing cross-reactivity when same species antibodies are simultaneously used. Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-Biotin Antibody has been tested in direct flow cytometry and immunocytochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.
DETAILS
- Form: Lyophilized powder
- Host: Mouse
- Clone: 6C1.
- Label: Biotin.
- Purity: Affinity purified on immobilized antigen.
- Target: IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel
- Comment: Contact Alomone Labs for technical support and product customization
- Gene Id: 3783
- Isotype: IgM
- Homology: Recognizes KCNN4 from human, rat, and mouse samples
- Is Toxin: No
- Sequence: Synthetic peptide mapping to the 3rd extracellular loop of human KCNN4
- Synonyms: IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel
- Accession: O15554
- Clonality: Monoclonal
- Lead Time: 1-2 Business Days
- Reactivity: Human|Rat|Mouse
- Formulation: PBS pH7.4, 1% BSA with 0.05% sodium azide
- Applications: FC|IC|IF|LCI
- Preservative: 1% BSA, 0.05% NaN3
- Reconstitution: 50 µl double distilled water (DDW).
- Blocking Peptide: NA
- Negative Control: NA
- Positive Control: NA
- Cited Application: IHC
- Immunogen Location: Extracellular loop
- Peptide Confirmation: NA
- Shipping And Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
- Immunogen Source Species: Human
- Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Antigen Preadsorption Control: NA
- Applications May Also Work In: FC|IC|IF|LCI
- Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
- Product Page Scientific Background: KCa3.1 (KCNN4, SK4) is a member of the Ca2+ activated K+ channel family that shares the characteristic of being activated by intracellular Ca2+. The channel has an intermediate conductance, is voltage insensitive and is activated by Ca2+ in the submicromolar range. The channel has a similar topology to that of KV channels, that is, six transmembrane domains and intracellular N- and C-termini. KCa3.1 is widely expressed in epithelial, endothelial and cells of hematopoietic origin. In erythrocytes (red blood cells) it has been identified as the molecular correlate of the so-called Gardos channel. The functional role of the channel is to set the cell membrane potential at negative values so as to aid in the electrochemical transport of other ions such as Cl- and Ca2+. Indeed, KCa3.1 has a key role in sustaining the Ca2+ influx in activated T lymphocytes and in regulating Cl- secretion from colon epithelium. Therefore, specific blockers of the KCa3.1 channel have been proposed for the treatment of several diseases including autoimmune diseases, secretory diarrhea and sickle cell anemia.
- Standard Quality Control Of Each Lot: Western blot analysis (unlabeled antibody, #ALM-051), and immunohistochemistry, Direct flow cytometry (labeled antibody).
- Application Dilutions Western Blot Wb: Contact Alomone for information
- Antibody Concentration After Reconstitution: 1 mg/ml
- Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: Contact Alomone for information