MOCS1 (NM_001075098) Human Tagged ORF Clone

DESCRIPTION:

MOCS1 (Myc-DDK-tagged)-Human molybdenum cofactor synthesis 1 (MOCS1), transcript variant 4

DETAILS

Mw: 42.9 kDa
Tag: Myc-DDK
Accn: NM_001075098
Type: Human Tagged ORF Clone
Refseq: NM_001075098.4
Status: In Stock*
Symbol: MOCS1
Vector: pCMV6-Entry
Category: cDNA Clones
Locus Id: 4337
Orf Size: 1155 bp
Synonyms: MIG11; MOCOD; MOCS1A; MOCS1B
Refseq Orf: 1158 bp
Uniprot Id: Q9NZB8
Refseq Size: 3027 bp
Availability: In Stock
Cytogenetics: 6p21.2
Gene Summary: Molybdenum cofactor biosynthesis is a conserved pathway leading to the biological activation of molybdenum. The protein encoded by this gene is involved in this pathway. This gene was originally thought to produce a bicistronic mRNA with the potential to produce two proteins (MOCS1A and MOCS1B) from adjacent open reading frames. However, only the first open reading frame (MOCS1A) has been found to encode a protein from the putative bicistronic mRNA, whereas additional splice variants are likely to produce a fusion between the two open reading frames. This gene is defective in patients with molybdenum cofactor deficiency, type A. A related pseudogene has been identified on chromosome 16. [provided by RefSeq, Nov 2017]
Chromatograms: CHROMATOGRAMS Sequencher program is needed, download here.
Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC217033 representing NM_001075098 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGCGGCGCGGCCACTGTCCCGGATGCTGCGGCGGCTTCTGAGGTCCAGCGCCCGGAGCTGCAGCTCAG GGGCTCCGGTGACCCAGCCCTGCCCCGGGGAGTCCGCGCGAGCTGCCTCGGAGGAGGTGTCCAGGCGGAG GCAGTTCCTGCGGGAGCATGCGGCCCCCTTCTCCGCCTTCCTCACAGACAGCTTCGGCCGGCAGCACAGC TACCTGCGGATCTCCCTCACAGAGAAGTGCAACCTCAGATGTCAGTACTGCATGCCCGAGGAGGGGGTCC CGCTGACCCCCAAAGCCAACCTGCTGACCACAGAGGAGATCCTGACCCTCGCCCGGCTCTTTGTGAAGGA AGGCATCGACAAGATCCGGCTCACAGGTGGAGAGCCGCTTATCCGGCCGGACGTGGTGGACATTGTGGCC CAGCTCCAGCGGCTGGAAGGGCTGAGAACCATAGGTGTTACCACCAATGGCATCAACCTGGCCCGGCTAC TGCCCCAGCTTCAGAAGGCTGGTCTCAGTGCCATCAACATCAGCCTGGACACCCTGGTGCCTGCCAAGTT TGAGTTCATTGTCCGCAGGAAAGGCTTCCACAAGGTCATGGAGGGCATCCACAAGGCCATCGAGCTGGGC TACAACCCTGTGAAGGTGAACTGTGTGGTGATGCGAGGCCTTAACGAGGATGAACTCCTGGACTTTGCGG CCTTGACTGAGGGCCTCCCCCTGGATGTGCGCTTCATAGAGTATATGCCCTTTGATGGCAACAAGTGGAA CTTCAAGAAGATGGTCAGCTATAAGGAGATGCTAGACACTGTCCGGCAGCAGTGGCCAGAGCTGGAGAAG GTGCCAGAGGAGGAATCCAGCACAGCCAAGGCCTTTAAAATCCCTGGCTTCCAAGGCCAGATCAGCTTCA TCACATCCATGTCTGAGCATTTCTGTGGGACCTGCAACCGCCTGCGAATCACAGCTGATGGGAACCTCAA GGTCTGCCTCTTTGGAAACTCTGAGGTATCCCTGCGGGATCACCTGCGAGCTGGGGCCTCTGAGCAGGAG CTGCTGAGAATCATTGGGGCTGCTGTGGGCAGGAAGAAGCGGCAGCATGCAGGCATGTTCAGTATTTCCC AGATGAAGAACCGGCCCATGATCCTCATCGGTGGG ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC217033 representing NM_001075098 Red =Cloning site Green =Tags(s) MAARPLSRMLRRLLRSSARSCSSGAPVTQPCPGESARAASEEVSRRRQFLREHAAPFSAFLTDSFGRQHS YLRISLTEKCNLRCQYCMPEEGVPLTPKANLLTTEEILTLARLFVKEGIDKIRLTGGEPLIRPDVVDIVA QLQRLEGLRTIGVTTNGINLARLLPQLQKAGLSAINISLDTLVPAKFEFIVRRKGFHKVMEGIHKAIELG YNPVKVNCVVMRGLNEDELLDFAALTEGLPLDVRFIEYMPFDGNKWNFKKMVSYKEMLDTVRQQWPELEK VPEEESSTAKAFKIPGFQGQISFITSMSEHFCGTCNRLRITADGNLKVCLFGNSEVSLRDHLRAGASEQE LLRIIGAAVGRKKRQHAGMFSISQMKNRPMILIGG TR TRPLEQKLISEEDLAANDILDYKDDDDK V
Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
E Coli Selection: Kanamycin (25 ug/mL)
Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Mammalian Cell Selection: Neomycin