DESCRIPTION

• Notes: The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria.
• Format: assay kit
• Shiptemp: -20°C
• Synonyms: NF-κB reporter, NFkappaB reporter, luciferase reporter, NF-κB reporter, NFkB reporter
• Warnings: Avoid freeze/thaw cycles.
• Category: Cell Based Assay Kit/Cell Signaling Pathway
• Background: NF-κB (Nuclear Factor-KappaB, NFkB) is a heterodimeric protein composed of different combinations of members of the Rel family of transcription factors. The NF-κB /Rel family of transcription factors (p50, p65, c-Rel, etc.) are involved in stress, immune, and inflammatory responses. In unstimulated cells, the NF-κB dimers are sequestered in the cytoplasm by inhibitory IkB proteins. Proinflammatory cytokines, LPS, growth factors, and antigen receptors activate I B kinase (IKK), which phosphorylates the I B proteins. Phosphorylation of IkB leads to its degradation, freeing NF-κB complexes to translocate to the nucleus, bind to NF-κB DNA response elements, and induce the transcription of the target genes.
• References: 1. Pessara, U., Koch, N. (1990). Mol Cell Biol. 10(8):4146-4154. 2. Baeuerle, P.A. (1998). Curr Biol. 8(1): R19-R22. Application Reference(s): 1. Transcription factors STAT6 and KLF4 implement macrophage polarization via the dual catalytic powers of MCPIP (2015) 2. Effects of angiotensin-(1-7) on the proliferation and collagen synthesis of arginine vasopressin-stimulated rat cardiac fibroblasts: role of mas receptor-calcineurin-NF-κB signaling pathway (2014)
• Description: The NF-κB Reporter kit is designed for monitoring the activity of the NF-κB signaling pathway in the cultured cells. The kit contains transfection-ready NF-κB luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of multimerized NF-κB responsive element located upstream of a minimal promoter. The NF-κB reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.
• Supplied As: Reporter: NF-κB luciferase reporter vector + constitutively-expressing Renilla luciferase vector Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector
• Unspsc Code: 41106513
• Unspsc Name: Reporter gene assay
• Applications: • Monitor NF-κB signaling pathway activity.• Screen for activators or inhibitors of NF-κB signaling or NF-κB pathway components.• Study effects of RNAi or gene overexpression on the activity of the NF-κB pathway.
• Product Type: Reporter Assay Kit
• Concentration: Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 60683-1, 60683-2, 60683-3, 79268, 60690, 60683, 79564
• Storage Stability: Stable at least 12 months from date of receipt, when stored as directed (-20°C)
• Scientific Category: Cell Signaling Pathway
• Instructions for Use: See data sheet insert for detailed transfection and assay protocols.