50261
HSP90α (C-Terminal Domain) TR-FRET Kit
BPS Bioscience
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DESCRIPTION
The HSP90α (C-Terminal Domain) TR-FRET assay kit is designed to measure the inhibition of
HSP90α binding to its protein target PPID (also known as Cyclophilin D or Cyp40) in a
homogeneous, 384 reaction format. This assay requires no time-consuming washing steps,
making it especially suitable for high throughput screening applications. The assay procedure is
straightforward and simple. A sample containing terbium-labeled donor, dye-labeled acceptor,
HSP90α, substrate, and an inhibitor is incubated for 2 hours. The protein-protein interaction is
then assayed by measuring the TR-FRET between the PPID and HSP90α using a fluorescence
reader.
DETAILS
- Notes: MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED: Fluorescent microplate reader capable of measuring Time Resolved Fluorescence ResonanceEnergy Transfer (TR-FRET)Adjustable micropipettor and sterile tips
- Uniprot: P07900
- Shiptemp: -80°C (dry ice)
- Synonyms: HSP90a, Heat Shock Protein 90a, HSP90alpha, HSP90α
- Warnings: Avoid freeze/thaw cycles.
- Category: Heat Shock Protein/Assay Kit
- References: Allan, R.K. et al. J. Biol. Chem. 2006 281(11): 7161-71
- Description: The HSP90α (C-Terminal Domain) TR-FRET assay kit is designed to measure the inhibition of HSP90α binding to its protein target PPID (also known as Cyclophilin D or Cyp40) in a homogeneous, 384 reaction format. This assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple. A sample containing terbium-labeled donor, dye-labeled acceptor, HSP90α, substrate, and an inhibitor is incubated for 2 hours. The protein-protein interaction is then assayed by measuring the TR-FRET between the PPID and HSP90α using a fluorescence reader.
- Methodology: TR-FRET
- Supplied As: Kit comes in a homogeneous, 384 reaction format
- Unspsc Code: 41121811
- Unspsc Name: Laboratory microchemistry kits
- Applications: Great for screening small molecular inhibitors for drug discovery and HTS applications.
- Product Type: Assay Kit
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 50290, 27501, 71095, 50317, 50316
- Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
- Scientific Category: Heat Shock Proteins/Assay Kits
- Instructions for Use: See assay kit datasheet for detailed protocol.