SARS-CoV-2 Spike S1+S2 (T19R, E156G, 157-158 deletion, L452R, T478K, D614G, P681R, D950N) Protein (ECD, His Tag)

DESCRIPTION

A DNA sequence encoding the SARS-CoV-2 Spike S1+S2 (YP_009724390.1, with mutations T19R, E156G, 157-158 deletion, L452R, T478K, D614G, P681R, D950N) (Met1-Pro1213) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant AY.3, AY.4) which emerged in the India.

DETAILS

Tag: C-His
Purity: > 90 % as determined by SDS-PAGE.
Source: Baculovirus-Insect Cells
Species: SARS-CoV-2
Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Activity: Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S1+S2 (T19R, E156G, 157-158 deletion, L452R, T478K, D614G, P681R, D950N) Protein (ECD, His Tag)(Cat:40589-V08B27), the EC50 is 200-1200ng/mL.
Molecule: Spike
Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
Lead Time: 1-3 days
Formulation: Lyophilized from sterile 20mM Tris, 300mM NaCl, 10% glycerol, pH 8.5.
Molecular Mass: The recombinant SARS-CoV-2 Spike S1+S2 consists of 1207 amino acids and predicts a molecular mass of 134.14 kDa.
Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Accession Number: YP_009724390.1
Predicted N Terminal: Val 16