RC233419
SOCS2 (NM_001270470) Human Tagged ORF Clone
Origene Technologies, Inc.
DESCRIPTION
SOCS2 (Myc-DDK tagged) - Homo sapiens suppressor of cytokine signaling 2 (SOCS2), transcript variant 5
DETAILS
- Mw: 22.2 kDa
- Tag: Myc-DDK
- Accn: NM_001270470
- Type: Human Tagged ORF Clone
- Refseq: NM_001270470.1, NP_001257399.1
- Status: In Stock*
- Symbol: SOCS2
- Vector: pCMV6-Entry
- Category: cDNA Clones
- Locus Id: 8835
- Orf Size: 594 bp
- Synonyms: CIS2; Cish2; SOCS-2; SSI-2; SSI2; STATI2
- Refseq Orf: 597 bp
- Uniprot Id: O14508
- Refseq Size: 2888 bp
- Availability: In Stock
- Cytogenetics: 12q22
- Gene Summary: This gene encodes a member of the suppressor of cytokine signaling (SOCS) family. SOCS family members are cytokine-inducible negative regulators of cytokine receptor signaling via the Janus kinase/signal transducer and activation of transcription pathway (the JAK/STAT pathway). SOCS family proteins interact with major molecules of signaling complexes to block further signal transduction, in part, by proteasomal depletion of receptors or signal-transducing proteins via ubiquitination. The expression of this gene can be induced by a subset of cytokines, including erythropoietin, GM-CSF, IL10, interferon (IFN)-gamma and by cytokine receptors such as growth horomone receptor. The protein encoded by this gene interacts with the cytoplasmic domain of insulin-like growth factor-1 receptor (IGF1R) and is thought to be involved in the regulation of IGF1R mediated cell signaling. This gene has pseudogenes on chromosomes 20 and 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]
- Chromatograms: CHROMATOGRAMS Sequencher program is needed, download here.
- Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC233419 ORF sequence Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGACCCTGCGGTGCCTTGAGCCCTCCGGGAATGGCGGGGAAGGGACGCGGAGCCAGTGGGGGACCGCGG GGTCGGCGGAGGAGCCATCCCCGCAGGCGGCGCGTCTGGCGAAGGCCCTGCGGGAGCTCGGTCAGACAGG ATGGTACTGGGGAAGTATGACTGTTAATGAAGCCAAAGAGAAATTAAAAGAGGCACCAGAAGGAACTTTC TTGATTAGAGATAGCTCGCATTCAGACTACCTACTAACAATATCTGTTAAAACATCAGCTGGACCAACTA ATCTTCGAATCGAATACCAAGACGGAAAATTCAGATTGGACTCTATCATATGTGTCAAATCCAAGCTTAA ACAATTTGACAGTGTGGTTCATCTGATCGACTACTATGTTCAGATGTGCAAGGATAAGCGGACAGGTCCA GAAGCCCCCCGGAACGGCACTGTTCACCTTTATCTGACCAAACCGCTCTACACGTCAGCACCATCTCTGC AGCATCTCTGTAGGCTCACCATTAACAAATGTACCGGTGCCATCTGGGGACTGCCTTTACCAACAAGACT AAAAGATTACTTGGAAGAATATAAATTCCAGGTA ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC233419 protein sequence Red =Cloning site Green =Tags(s) MTLRCLEPSGNGGEGTRSQWGTAGSAEEPSPQAARLAKALRELGQTGWYWGSMTVNEAKEKLKEAPEGTF LIRDSSHSDYLLTISVKTSAGPTNLRIEYQDGKFRLDSIICVKSKLKQFDSVVHLIDYYVQMCKDKRTGP EAPRNGTVHLYLTKPLYTSAPSLQHLCRLTINKCTGAIWGLPLPTRLKDYLEEYKFQV TR TRPLEQKLISEEDLAANDILDYKDDDDK V
- Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
- Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
- Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
- E Coli Selection: Kanamycin (25 ug/mL)
- Protein Families: Druggable Genome
- Protein Pathways: Insulin signaling pathway, Jak-STAT signaling pathway, Type II diabetes mellitus
- Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
- Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
- Mammalian Cell Selection: Neomycin