ALR-062-F_15 mcl ALR-062-F_50 mcl ALR-062-F_50 mcl ALR-062-F_50 mcl ALR-062-F_50 mcl +1
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Anti-CLEC7A/Dectin-1 (extracellular)-FITC Antibody
Alomone Labs
DESCRIPTION
Anti-CLEC7A/Dectin-1 (extracellular) Antibody (#ALR-062) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize CLEC7A from mouse and rat samples. The antibody will not recognize CLEC7A from human samples. Anti-CLEC7A/Dectin-1 (extracellular)-FITC Antibody (#ALR-062-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize CLEC7A from mouse and rat samples. The antibody will not recognize CLEC7A from human samples.
DETAILS
- Form: Lyophilized powder
- Host: Rabbit
- Clone: NA
- Label: Fluorescein isothiocyanate (FITC).
- Purity: Affinity purified on immobilized antigen.
- Target: C-Type Lectin Domain Family 7 Member A, Dendritic Cell-Associated C-Type Lectin 1, DC-Associated C-Type Lectin 1, Beta-Glucan Receptor, C-Type Lectin Superfamily Member 12, CLECSF12, CD369, SCARE2
- Comment: Contact Alomone Labs for technical support and product customization
- Gene Id: 56644
- Isotype: Rabbit IgG
- Homology: Rat - 15 out of 16 amino acid residues identicalNot recommended for human samples
- Is Toxin: No
- Sequence: (C)RNKENHKPTESSLDEK, corresponding to amino acid residues 87 - 102 of mouse CLEC7A
- Synonyms: C-Type Lectin Domain Family 7 Member A, Dendritic Cell-Associated C-Type Lectin 1, DC-Associated C-Type Lectin 1, Beta-Glucan Receptor, C-Type Lectin Superfamily Member 12, CLECSF12, CD369, SCARE2
- Accession: Q6QLQ4
- Clonality: Polyclonal
- Lead Time: 1-2 Business Days
- Reactivity: Rat|Mouse
- Formulation: PBS pH7.4, 1% BSA with 0.05% sodium azide
- Applications: FC|LCI
- Preservative: 1% BSA, 0.05% NaN3
- Reconstitution: 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
- Blocking Peptide: BLP-LR062
- Negative Control: RIC-001-F
- Positive Control: NA
- Immunogen Location: Extracellular, C-terminus.
- Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
- Shipping and Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
- Immunogen Source Species: Mouse
- Storage After Reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
- Applications May Also Work In: FC|LCI
- Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
- Product Page - Scientific Background: Dectin-1, encoded by CLEC7A, is a small transmembrane receptor that is a member of the C-type lectin family of pattern-recognition receptors (PRRs)1,2. As this receptor specifically recognizes and binds to β-glucans polysaccharides derived from the cell walls of fungi and yeast, it has been characterized for its role in antifungal immunity3.Dectin-1 is predominately expressed on macrophages, dendritic cells, and neutrophils4,5. Upon ligation of β-glucan, Dectin-1 recruits the adaptor protein CARD9 to phosphorylate spleen tyrosine kinase (Syk) which in turn initiates a signaling cascade which induces phagocytosis and leads to the activation of nuclear factor-κB (NF-κB) and MAPK6. The Dectin-1 Syk mediated pathway has been demonstrated to be activated and involved in the alternative activation of macrophages as Dectin-1 was upregulated in vitro within 4 hours when stimulated with IL-4 and IL-13, cytokines which induce alternative activation of macrophages7. Moreover, the activated Syk pathway has also been implicated in renal interstitial fibrotic disease where it was demonstrated that suppressing Syk in an experimental mouse model of renal fibrosis inhibits the progression of kidney fibrosis, shown by a reduction in mRNA expression of Arginase-1 (murine M2 macrophage marker)8.While the Dectin-1 pathway has been shown to be involved in the bleomycin-induced model of skin fibrosis, Dectin-1 signaling and receptor expression has yet to be investigated in the context of pulmonary fibrosis9.
- Standard Quality Control of Each Lot: Western blot analysis (unlabeled antibody, #ALR-062), and direct flow cytometry (labeled antibody).
- Application Dilutions Western Blot Wb: Contact Alomone for information
- Antibody Concentration After Reconstitution: 1 mg/ml
- Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: Contact Alomone for information